| H2O2对陈化马铃薯切片抗氰呼吸的诱导作用研究 |
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| 引用本文： | 梁五生,梁厚果,李德葆.H2O2对陈化马铃薯切片抗氰呼吸的诱导作用研究[J].浙江大学学报(农业与生命科学版),2002,28(5):525-528. |
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| 作者姓名： | 梁五生 梁厚果 李德葆 |
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| 作者单位： | 1. 浙江大学,生物技术研究所,浙江,杭州,310029
2. 四川大学,生物系,四川,成都,610064 |
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| 基金项目： | 国家自然科学基金资助项目(39900010) |
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| 摘 要： | 研究了H2O2对陈化马铃薯切片抗氰呼吸的诱导作用.用切片和纯化线粒体进行测定的结果均表明外源H2O2(5.0 mmol/L)处理对陈化马铃薯切片的总呼吸只有微弱的影响,但却可以明显诱导切片的抗氰呼吸,并显著提高抗氰呼吸对总呼吸的贡献.应用交替氧化酶的单克隆抗体进行Western杂交的结果表明,H2O2处理可以增强陈化马铃薯切片中交替氧化酶的表达,表明H2O2对抗氰呼吸的诱导作用与其时交替氧化酶表达的诱导有关.抗氰呼吸可能参与了H2O2调控植物抗病防御反应的作用机制.
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| 关 键 词： | 抗氰呼吸 H2O2 马铃薯 陈化 抗病作用 |
| 文章编号： | 1008-9209(2002)05-0525-04 |
Studies on the induction of cyanide-resistant respiration by H2O2 in aging potato tuber slices |
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| LIANG Wu sheng ,LIANG Hou guo ,LI De bao.Studies on the induction of cyanide-resistant respiration by H2O2 in aging potato tuber slices[J].Journal of Zhejiang University(Agriculture & Life Sciences),2002,28(5):525-528. |
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| Authors: | LIANG Wu sheng LIANG Hou guo LI De bao |
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| Institution: | LIANG Wu sheng 1,LIANG Hou guo 2,LI De bao 1 |
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| Abstract: | The influences of exogenous H 2O 2 on the cyanide resistant respiration in aging potato ( Solanum tuberosum L ) tuber slices were investigated Results showed that H 2O 2 (5 0 mmol/L) had a little influence on the total respiration ( V t) of the slices,but showed a significant inducing effect on the cyanide resistant respiration ( V alt ) and its contribution to total respiration ( V alt / V t) in the slices during 24 h of aging process The significant inducing effects of H 2O 2 on the cyanide resistant respiration were also found in mitochondria purified from the aging potato slices Western blotting with monoclonal antibody against alternative oxidase showed that H 2O 2 treatment increased the expression of alternative oxidase in aging potato tuber slices, which implies that the induction of cyanide resistant respiration by H 2O 2 was related to alternative oxidase expression |
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| Keywords: | cyanide resistant respiration H 2O 2 Solanum tuberosum L aging disease resistance |
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