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| 山东省牛源金黄色葡萄球菌耐药谱分析及mecA耐药基因检测 | |
| 引用本文： | 李秀梅,杨宏军,肖红,王慧红,王青泉,何洪彬,宋玲玲,侯佩莉,田晶.山东省牛源金黄色葡萄球菌耐药谱分析及mecA耐药基因检测[J].山东农业科学,2012,44(3):13-16. |
| 作者姓名： | 李秀梅 杨宏军 肖红 王慧红 王青泉 何洪彬 宋玲玲 侯佩莉 田晶 |
| 作者单位： | 1. 大连工业大学,辽宁大连,116034 2. 山东省农业科学院奶牛研究中心,山东济南,250100 3. 潍坊诺达药业有限公司,山东潍坊,261000 4. 山东泉港药业有限公司,山东济南,250000 5. 东北农业大学,黑龙江哈尔滨,150000 |
| 基金项目： | 山东省中青年科学家基金(BS2009NY002);现代农业(奶牛)产业技术体系科学家岗位;山东省科技攻关课题(2009GG20002032) |
| 摘 要： | 采用纸片扩散法测定金黄色葡萄球菌(以下简称金葡菌)对6种抗生素的敏感性。结果表明,在测定的121株金葡菌中,耐苯唑西林金葡菌(MRSA)为26株,占21.5%;对苯唑西林敏感菌(MSSA)为93株,占76.9%;中介菌株为2株,占1.6%。金葡菌对6种抗生素耐药率最低的是头孢噻肟为2株,占1.6%。mecA基因的PCR扩增结果显示:所有的MSSA mecA基因均阴性,中介株mecA基因阳性1株,而MRSA中mecA基因阳性株为9株。MSSA对大部分抗生素仍保持良好的敏感性,而MRSA表现为多重耐药性,PCR技术可以作为快速检测金葡菌耐药基因的有效方法。 |
| 关 键 词： | 金黄色葡萄球菌 耐药性 耐药基因 |
Analysis of Antibiotics Resistance of Staphylococcus aureus Isolated from Bovine Mastitis in Shandong Province and Detection of mecA Gene |
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| LI Xiu-mei , YANG Hong-jun , XIAO Hong , WANG Hui-hong , WANG Qing-quan , HE Hong-bin , SONG Ling-ling , HOU Pei-li , TIAN Jing.Analysis of Antibiotics Resistance of Staphylococcus aureus Isolated from Bovine Mastitis in Shandong Province and Detection of mecA Gene[J].Shandong Agricultural Sciences,2012,44(3):13-16. | |
| Authors: | LI Xiu-mei YANG Hong-jun XIAO Hong WANG Hui-hong WANG Qing-quan HE Hong-bin SONG Ling-ling HOU Pei-li TIAN Jing |
| Institution: | 1(1.Dalian Polytechnic University,Dalian 116034,China;2.Dairy Cattle Research Center of Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Weifang Noted Pharmaceutical Co.,Ltd.,Weifang 261000,China; 4.Shandong Quangang Pharmaceutical Co.,Ltd.,Jinan 250000,China; 5.Northeast Agricultural University,Harbin 150000,China) |
| Abstract: | The susceptibility of 121 strains of Staphylococcus aureus isolated from bovine mastitis to six kinds of antibiotics was determined by disk diffusion method.The results showed that 26 strains occupied 21.5% were methicillin-resistant Staphylococcus aureus(MRSA),while 93 strains occupied 76.9% were susceptible to oxacillin(MSSA),and 2 strains were intermediary.There were only 2 strains of Staphylococcus aureus had the lowest resistant rate to cefotaxime,which occupied 1.6%.The PCR results showed that the mecA gene of all oxacillin-sensitive strains were negative,while that of one intermediary strain and 9 MRSA strains was positive.The MSSA strains still maintained good susceptibility to most of antibiotics,but the MRSA strains showed multi-drug resistance.PCR technology can be used as a rapid and effective way to detect the drug resistant genes of Staphylococcus aureus. |
| Keywords: | Staphylococcus aureus Resistance Drug resistant gene |
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