| 红花石榴二氢黄酮醇还原酶(DFR)基因cDNA片段克隆及序列分析 |
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| 引用本文： | 招雪晴,苑兆和,陶吉寒,尹艳雷,冯立娟.红花石榴二氢黄酮醇还原酶(DFR)基因cDNA片段克隆及序列分析[J].山东农业科学,2012,44(2):1-4. |
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| 作者姓名： | 招雪晴 苑兆和 陶吉寒 尹艳雷 冯立娟 |
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| 作者单位： | 山东省果树研究所,山东泰安,271000 |
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| 基金项目： | 山东省自然基金，国家外专局项目，山东省农业科技成果转化项目 |
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| 摘 要： | 二氢黄酮醇还原酶(DFR)是植物花色苷生物合成途径中的关键酶。以红花石榴"泰山红"的花瓣总RNA为模板,利用简并引物,采用RT-PCR方法,扩增出一条长446 bp的cDNA片段,序列分析结果表明,该cDNA片段与GenBank中的甜樱桃、葡萄等物种的DFR氨基酸同源性为78%～81%,说明该二氢黄酮醇还原酶片段为石榴花DFR基因的cDNA片段,GenBank登录号为JN316028。
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| 关 键 词： | 石榴 DFR基因 克隆 序列分析 |
Cloning and Sequence Analysis of cDNA Fragment of DFR Gene from Red - flower Pomegranate |
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| ZHAO Xue-qing
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YUAN Zhao-he
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TAO Ji-han
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YIN Yan-lei
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FENG Li-juan.Cloning and Sequence Analysis of cDNA Fragment of DFR Gene from Red - flower Pomegranate[J].Shandong Agricultural Sciences,2012,44(2):1-4. |
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| Authors: | ZHAO Xue-qing YUAN Zhao-he TAO Ji-han YIN Yan-lei FENG Li-juan |
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| Institution: | (Shandong Institute of Pomology,Taian 271000,China) |
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| Abstract: | Dihydroflavonol 4-reductase(DFR)is the key enzyme in the biosynthesis of anthocyanidins.With the total RNA from red-flower pomegranate "Taishanhong" as template and using degenerate primers,a new cDNA fragment of 446 bp was amplified by RT-PCR.The sequence analysis demonstrated that the deduced amino acid sequence shared 78%～81% homology with that of sweet cherry,grape and etc.These results suggested that the cloned cDNA fragment was from DFR gene of red-flower pomegranate,and its accession number in GenBank was JN316028. |
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| Keywords: | Pomegranate DFR gene Clone Sequence analysis |
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