| 茄子组织培养再生体系研究初报 |
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| 引用本文： | 俞琼,陈利,刘鹏,徐根娣.茄子组织培养再生体系研究初报[J].广东农业科学,2007(2):24-26. |
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| 作者姓名： | 俞琼 陈利 刘鹏 徐根娣 |
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| 作者单位： | 1. 浙江师范大学初阳学院,浙江,金华,321004;浙江师范大学植物学实验室,浙江,金华,321004
2. 浙江师范大学植物学实验室,浙江,金华,321004 |
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| 基金项目： | 浙江省自然科学基金
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浙江省金华市重点科技基金 |
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| 摘 要： | 以茄子的子叶和下胚轴为外植体,研究了不同激素组合对外植体分化的影响,建立茄子下胚轴高效再生体系,并初步探讨了温度对植株再分化的影响.结果表明:下胚轴不定芽诱导的最适培养基为MS NAA 0.2 mg/L 6-BA 2.5 mg/L,芽诱导率为46%;子叶不定芽诱导的最适培养基为MS NAA 0.2 mg/L KT 2.0 mg/L,芽诱导率为37%.
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| 关 键 词： | 茄子 子叶 下胚轴 组织培养 植株再生 |
| 文章编号： | 1004-874X(2007)02-0024-03 |
| 修稿时间： | 2006-09-23 |
Study on regeneration system by tissue culture of eggplant |
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| YU Qiong,CHEN Li,LIU Peng,XU Gen-di.Study on regeneration system by tissue culture of eggplant[J].Guangdong Agricultural Sciences,2007(2):24-26. |
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| Authors: | YU Qiong CHEN Li LIU Peng XU Gen-di |
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| Institution: | 1. Chu Yang Honor College, Zhejiang Normal University, Jinhua 321004, China ; 2. Key Laboratory of Botany, Zhejiang Normal University, Jinhua 321004, China |
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| Abstract: | The influence of different growth regulating substances on explants differentiation was studied with the cotyledon and hypocotyls explants of eggplants.High efficient regeneration systems of cotyledon and hypocotyl were established.The influence of temperature on the regeneration was discussed.The results showed that the best medium for initiation of adventitious bud of hypocotyl was MS medium supplemented with NAA 0.2 mg/L and 6-BA 2.5 mg/L.The propagation ratio of bud was 46%.The best medium for initiation of adventitious bud of cotyledon was MS medium supplemented with NAA 0.2 mg/L and KT 2.0 mg/L.The propagation ratio of buds wa 37%. |
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| Keywords: | eggplant cotyledon hypocotyl tissue culture plant regeneration |
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