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| 甘薯sporamin蛋白对3T3-L1前脂肪细胞分化和增殖的影响 | |
| 引用本文： | 熊志冬,李鹏高,邓乐,唐玉平,木泰华.甘薯sporamin蛋白对3T3-L1前脂肪细胞分化和增殖的影响[J].中国农业科学,2009,42(8):2892-2898. |
| 作者姓名： | 熊志冬 李鹏高 邓乐 唐玉平 木泰华 |
| 作者单位： | 1. 中国农业科学院农产品加工研究所,北京,100193 2. 首都医科大学公共卫生与家庭医学学院,北京,100069 3. 首都医科大学燕京医学院,北京,100069 |
| 基金项目： | 国家高技术研究发展计划(863计划)，现代农业产业技术体系建设专项基金 |
| 摘 要： | 【目的】探讨甘薯sporamin蛋白对3T3-L1前脂肪细胞分化与增殖的影响,为开发预防和治疗肥胖、糖尿病的保健食品提供理论依据。【方法】采用硫酸铵沉淀、离子交换、凝胶过滤层析的方法对‘55-2’甘薯中的sporamin蛋白进行分离纯化。然后,以黄连素为阳性对照,用不同浓度sporamin（0、0.025、0.125、0.250、0.500、1.000 mg?ml-1）处理3T3-L1前脂肪细胞。采用油红O染色和比色定量检测细胞内脂肪生成及细胞分化程度,以MTT法检测细胞的增殖。【结果】经离子交换、凝胶层析可纯化出高纯度的sporamin蛋白A和B（相对分子量分别为31 kD和22 kD）。与空白相比,用不同浓度的sporamin蛋白处理后,3T3-L1前脂肪细胞的分化受到明显抑制。当sporamin蛋白浓度增至0.500 mg?ml-1时,脂滴生成量明显减少,洗脱液吸光度值降至最低为0.35（P＜0.05）。此外,高浓度的sporamin蛋白能有效地抑制3T3-L1前脂肪细胞的增殖,且随着处理时间延长抑制效果更加明显(P＜0.05).【结论】甘薯sporamin蛋白能抑制3T3-L1前脂肪细胞的分化和增殖,具有潜在的减肥作用。 |
| 关 键 词： | 甘薯 sporamin 3T3-L1前脂肪细胞 |
| 收稿时间： | 2008-12-16; |
Differentiation-and Proliferation-Inhibitory Effects of Sporamin from Sweetpotato on 3T3-L1 Preadipocytes |
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| XIONG Zhi-dong,LI Peng-gao,DENG Le,TANG Yu-ping,MU Tai-hua.Differentiation-and Proliferation-Inhibitory Effects of Sporamin from Sweetpotato on 3T3-L1 Preadipocytes[J].Scientia Agricultura Sinica,2009,42(8):2892-2898. | |
| Authors: | XIONG Zhi-dong LI Peng-gao DENG Le TANG Yu-ping MU Tai-hua |
| Institution: | (Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Sciences) |
| Abstract: | 【Objective】 The aim of this study is to investigate the effect of different concentrations of sporamin on differentiation and proliferation of 3T3-L1 preadipocytes, providing a theoretical basis for the development of prevention and treatment of obesity and anti-diabetes reagent in food industry. 【Method】 Isolation and purification of sporamin from sweet potato 55-2 was performed by ammonium sulphate precipitation in combination with ion-exchange-, gel filtration chromatography. With berberine as a positive control, different concentrations of sporamin (0, 0.025, 0.125, 0.250, 0.500 and 1.000 mg?ml-1, respectively) were used to treat 3T3-L1 preadipocytes. Intracellular fat accumulation and the degree of adipogenesis were observed using oil red O staining and quantified by colorimetry. Preadipocytes differentiation was measured by MTT spectrophotometry. 【Result】 Two sporamin proteins which were separated into sporamin A (31 kD) and sporamin B (22 kD) could be purified by ion-Exchange-, gel filtration chromatography. After being treated by different sporamin concentrations, the differentiation of 3T3-L1 preadipocytes was significantly inhibited compared with control. When the concentration of sporamin solution was 0.500 mg?ml-1, the accumulation of lipid droplets within the cells were obviously decreased and the optical density (OD) value of the solution from destained Oil Red O reached to 0.35, which was the lowest value (P＜0.05). The proliferation of 3T3-L1 preadipocytes was significantly inhibited by treating at higher sporamin concentration. In addition, the restrictive effect was more obvious with the prolonging of treatment time (P＜0.05). 【Conclusion】Differentiation and proliferation of 3T3-L1 preadipocytes could be inhibited significantly by addition of higher concentration sporamin. It is, therefore, suggested that sporamin is effective for weight loss. |
| Keywords: | sporamin |
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