| Bacillus subtilis fmbR抗菌物质的分离和鉴定 |
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| 引用本文： | 别小妹,陆兆新,吕凤霞,赵海珍,杨胜远,孙力军.Bacillus subtilis fmbR抗菌物质的分离和鉴定[J].中国农业科学,2006,39(11):2327-2334. |
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| 作者姓名： | 别小妹 陆兆新 吕凤霞 赵海珍 杨胜远 孙力军 |
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| 作者单位： | 南京农业大学食品科技学院 |
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| 摘 要： | 【目的】对Bacillus subtilis fmbR产生的抗菌物质进行分离和鉴定研究，以确定抗菌物质的组成和结构。【方法】采用HPLC和TLC层析对Bacillus subtilis fmbR抗菌物质进行分离纯化，通过ESI-MS和MALDI-MS分析对抗菌物质的组成和结构进行初步鉴定。【结果】HPLC层析表明了Bacillus subtilis fmbR抗菌物质含有保留时间与surfactin相似的成分。TLC层析和原位酸解证明了Bacillus subtilis fmbR抗菌物质含有闭合肽键类的物质，其中之一为相对迁移率Rf与标样surfactin相近的组分。采用ESI-MS分析检测到Bacillus subtilis fmbR抗菌物质含有分子量与surfactinA相同的m/z1009.1、m/z1023.2 和m/z1037.0等3种同系物；通过MALDI-MS分析获得M+H]+为m/z 3403.95抗菌物质，该物质分子量与Bacillus subtilis 168产生的细菌素subtilosin的m/z3403.3 相同。【结论】Bacillus subtilis fmbR抗菌物质由C13～C15的3种surfactinA同系物和一种羊毛硫抗生素subtilosin组成。
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| 关 键 词： | 枯草芽孢杆菌（Bacillus subtilis fmbR） 抗菌物质 分离 鉴定 |
| 收稿时间： | 2006-03-03 |
| 修稿时间： | 2006-03-032006-07-28 |
Isolation and Identification of the Antimicrobial Substance Produced by Bacillus subtilis fmbR |
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| BIE Xiao-mei,LU Zhao-xin,L Feng-xia,ZHAO Hai-zhen,YANG Sheng-yuan,SUN Li-jun.Isolation and Identification of the Antimicrobial Substance Produced by Bacillus subtilis fmbR[J].Scientia Agricultura Sinica,2006,39(11):2327-2334. |
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| Authors: | BIE Xiao-mei LU Zhao-xin L Feng-xia ZHAO Hai-zhen YANG Sheng-yuan SUN Li-jun |
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| Institution: | BIE Xiao-mei,LU Zhao-xin,L(U) Feng-xia,ZHAO Hai-zhen,YANG Sheng-yuan,SUN Li-jun |
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| Abstract: | Isolation and purification of lipopeptides from Bacillus subtilis fmbR was studied in this paper. With HPLC methods, it was determined that the antimicrobial substance was composed of many components, and one of tham had the similar retaining time to surfactin. In addition, the antimicrobial substance was proved to include the closed circle peptide band by TLC, and one of them had the similar moving rate to surfactin. Furthermore, ESI-MS analysis showed that the antimicrobial substance contained three homologues of surfactin, such as m/z1009.1,m/z1023.2 and m/z1037.0. MALDI-TOF-MS analysis indecated it also contained the M+H]+ ion peak at m/z 3403.95, which is very similar to molecular mass of subtilosin produced by Bacillus subtilis168. |
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| Keywords: | Bacillus subtilis fmbR Antimicrobial substance Isolation Identification |
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