| 表达新城疫HN和F基因重组禽痘病毒的免疫效力测定 |
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| 引用本文： | 王月明,SUN Xiao-jun,刘玉山,LIU Wei,于可响.表达新城疫HN和F基因重组禽痘病毒的免疫效力测定[J].水禽世界,2008(8). |
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| 作者姓名： | 王月明 SUN Xiao-jun 刘玉山 LIU Wei 于可响 |
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| 作者单位： | 1. 山东省农业科学院,济南,250000
2. 山东省农业科学院家禽研究所,济南,250023 |
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| 摘 要： | 将8日龄SPF鸡随机分组.分别接种rFPVHN、rFPVF、rFPVNHF重组禽痘病毒,接种量为每只鸡105PFU,另一组群用La Sota活苗经点眼、滴鼻接种10<'6>EID<,50>.于免疫后6d、12d和18d测定HI和微量中和抗体.结果发现,3种重组病毒均能刺激鸡群产生抗新城疫病毒特异抗体,但抗体产生时间比La Sota活苗缓慢,且抗体滴度亦低.然而共表达HN和F的rFPVNHF免疫鸡群的中和抗体水平明显比rFPVHN和rFPVF组群高.表明rFPVNHF的保护性免疫更优于HN或F单基因重组禽痘病毒.
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| 关 键 词： | 禽痘病毒 新城疫病毒 重组病毒:免疫效力 |
Immune Efficacy Evaluation of recombinant fowlpox virus expressing hemagglutinin-neuraminidase and fusion protein of Newcastle disease virus |
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| WANG Yue-ming,SUN Xiao-jun,LIU Yu-shan,LIU Wei,YU Ke-xiang.Immune Efficacy Evaluation of recombinant fowlpox virus expressing hemagglutinin-neuraminidase and fusion protein of Newcastle disease virus[J].Poultry Science,2008(8). |
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| Authors: | WANG Yue-ming SUN Xiao-jun LIU Yu-shan LIU Wei YU Ke-xiang |
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| Institution: | 1.Shandong academy of agricultural sciences;Jinan 250000;China;2.Institute of poultry science;Shandong academy of agricultural sciences;Jinan 250023;China |
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| Abstract: | Eight-day-old Specific pathogen free chickens were randomly grouped,and respectively inoculated with recombinant fowlpox virus rFPVHN expressing hemagglutinin-neuraminidase of Newcastle disease virus(NDV),rFPVF expressing fusion protein of NDV,rFPVNHF co-expressing hemagglutinin-neuraminidase and fusion protein of NDV by 105 plaque forming unit(PFU)each chicken,and another group were immunized with Newcastle disease virus La Sota strain live vaccine by eyedrop and rhinovaccination. Hemagglutinin inhibition ... |
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| Keywords: | Fowlpox virus Newcastle disease virus Recombinant virus Immune efficacy |
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