| 家蚕线粒体基因组1.8kb片段的克隆及序列分析 |
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| 引用本文： | 廖顺尧,刘运强,鲁成,周泽扬,向仲怀.家蚕线粒体基因组1.8kb片段的克隆及序列分析[J].蚕业科学,2002,28(1):26-30. |
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| 作者姓名： | 廖顺尧 刘运强 鲁成 周泽扬 向仲怀 |
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| 作者单位： | 西南农业大学蚕桑丝绸学院,农业部蚕桑学重点开放实验室,重庆,400716 |
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| 基金项目： | 国家自然科学基金资助项目 (编号 :3 0 170 719) |
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| 摘 要： | 克隆并测定了家蚕 (Bombyxmori)线粒体基因组 1781bp的EcoRⅠ和HindⅢ双酶切片段序列 ,根据序列同源性比较 ,推测该DNA片段包括 :ND1基因、16SrRNA基因 3′端、tRNALeu(UAG)基因和一个尚待确定的tRNA基因。家蚕与果蝇ND1基因序列同源性约为 81 85 % ,16SrRNA基因 3′端序列同源性约为 74 5 %。在家蚕、果蝇、蜜蜂、蝗虫、卤虫和人 6个物种中 ,线粒体ND1编码的疏水性氨基酸比例较稳定 ,显示了ND1基因的保守性。对上述 6个物种ND1基因序列和 16SrRNA基因 3′端序列进行系统进化分析 ,构建了系统进化树。
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| 关 键 词： | 家蚕 线粒体基因组 18kb片段 基因组成 基因结构 系统进化 |
| 修稿时间： | 2001年3月14日 |
Clone and Analysis of a 1.8kb Fragment Sequence of Bombyx mori Mitochondrial Genome |
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| Liao Shunyao,Liu Yunqiang,Lu Cheng,Zhou Zeyang,Xiang Zhonghuai.Clone and Analysis of a 1.8kb Fragment Sequence of Bombyx mori Mitochondrial Genome[J].Acta Sericologica Sinica,2002,28(1):26-30. |
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| Authors: | Liao Shunyao Liu Yunqiang Lu Cheng Zhou Zeyang Xiang Zhonghuai |
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| Abstract: | A 1,781bp fragment of Bombyx mori mitochondrial genome derived from Eco RⅠ and Hin dⅢ double digestion was cloned and sequenced.The sequence contains ND1 gene,3′ end of 16S rRNA gene,tRNA Leu gene and an unconfirmed tRNA gene.The homology of ND1 gene between B.mori and D.melanogaster is about 81.85%,and that of 3′ end sequence of 16S rRNA gene between the two species is about 74.5%.The content of the hydrophobic amino acids of ND1 is stable among 6 species(i.e. H.sapiens,A.franciscan,L.migratoria,D.melanogaster,B.mori and A.mellifera) ,which indicates ND1 gene is conservative in different species.The molecular phylogenetic trees of ND1 gene and 3′ end of 16S rRNA gene of the 6 species were constructed by Maximum Likelihood Method(ML). |
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| Keywords: | Bombyx mori Mitochondrial genome 1 8kb fragment Gene composition Gene structure Phylogenesis |
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