| 盐胁迫条件下不同耐盐棉花miRNA差异表达研究 |
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| 引用本文： | 李春贺,阴祖军,刘玉栋,沈法富.盐胁迫条件下不同耐盐棉花miRNA差异表达研究[J].山东农业科学,2009(7):12-17. |
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| 作者姓名： | 李春贺 阴祖军 刘玉栋 沈法富 |
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| 作者单位： | 山东农业大学农学院,山东,泰安,271018 |
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| 摘 要： | 植物miRNAs在基因表达、生长发育和抵抗胁迫等方面有十分重要的作用。本试验以耐盐棉花品系山农91—11和盐敏感棉花品种鲁棉6号为试材，利用miRNA基因芯片杂交技术，分析盐胁迫条件下棉花幼苗miRNA的差异表达。结果表明：在盐胁迫条件下，共有27个miRNAs在山农91—11和鲁棉6号之间差异表达，其中山农91—11比鲁棉6号表达上调的miRNAs有11个，其中功能已知的10个分别属于miR156、miR164、miR167、miR397和miR399家族，表达下调的miRNAs有16个，其中功能已知的2个属于miR156、miR1l72家族。对这些miRNA家族作用的靶基因进行分析表明，这些miRNAs参与植物逆境条件下的信号传导，可能对棉花耐盐机制有重要作用。
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| 关 键 词： | 棉花 miRNA芯片 盐胁迫 差异表达 |
Differential Expression of miRNA in Different Salt-tolerant Cotton Varieties under Salt Stress |
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| LI Chun-he,YIN Zu-jun,LIU Yu-dong,SHEN Fa-fu.Differential Expression of miRNA in Different Salt-tolerant Cotton Varieties under Salt Stress[J].Shandong Agricultural Sciences,2009(7):12-17. |
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| Authors: | LI Chun-he YIN Zu-jun LIU Yu-dong SHEN Fa-fu |
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| Institution: | Agronomy College;Shandong Agricultural University;Taian 271018;China |
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| Abstract: | Plant miRNAs have important impacts on gene expression,growth and development,resistance to stress and so on.This study used the salt-tolerant cotton line Shannong 91-11 and salt-sensitive cultivar Lumian 6 as the research materials to detect the differential expression of cotton miRNAs under salt stress by miRNA microarray.The results showed that there were 27 miRNAs expressing differently between Shannong 91-11 and Lumian 6 under salt stress;compared with Lumian 6,11 miRNAs up-regulated their expressions,... |
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| Keywords: | Cotton miRNA chip Salt stress Differential expression |
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