| 注射多巴胺对中华绒螯蟹打斗行为的影响 |
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| 引用本文： | 代亮,李晓东,李艺,刘汉堤,曹萌,郑岩,孙娜.注射多巴胺对中华绒螯蟹打斗行为的影响[J].水产科学,2020,39(3):324-331. |
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| 作者姓名： | 代亮 李晓东 李艺 刘汉堤 曹萌 郑岩 孙娜 |
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| 作者单位： | 大连海洋大学,辽宁大连116023;盘锦光合蟹业有限公司研发中心,辽宁省中华绒螯蟹育种重点实验室,辽宁光合河蟹专业技术创新平台,企业工程技术研究中心,辽宁盘锦124200;盘锦光合蟹业有限公司研发中心,辽宁省中华绒螯蟹育种重点实验室,辽宁光合河蟹专业技术创新平台,企业工程技术研究中心,辽宁盘锦124200;沈阳农业大学,辽宁沈阳110866 |
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| 基金项目： | 国家虾蟹产业技术体系建设任务书项目(CARS-48);辽宁省重点研发计划指导项目(2018203002);辽宁省科技厅项目(XLYC1802053 b). |
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| 摘 要： | 选同一家系规格相似、健康有活力的中华绒螯蟹新品种“光合1号”1龄雄蟹100只,分为4组,放入直径20 cm、高40 cm PE管(底部均匀钻12个直径为5 mm的孔洞)中,PE管置于120 L水槽中。用2个不透明烧杯将2只中华绒螯蟹分别倒扣于试验容器中,置于PE管两侧1 h。在中华绒螯蟹第三步足的动脉膜中分别注射20μL生理盐水(对照组),10^-8 mol、10-7 mol和10-6 mol的多巴胺液,利用闭路摄像头记录4 h内2只中华绒螯蟹打斗时长、次数、打斗分数及胜负关系等指标。打斗结束后,迅速取出中华绒螯蟹血淋巴,用ELISA酶联免疫法测定多巴胺含量。试验结果显示,对照组中华绒鳌蟹打斗时间最长,与各注射组差异显著(P<0.05);注射组中,随注射量的增加,打斗时长下降。对照组中华绒螯蟹打斗强度低于10^-8 mol组,高于其他注射组,与各注射组间差异显著(P<0.05)。注射组中,随注射多巴胺量升高,打斗强度下降,10^-8 mol组打斗强度最高,各组间差异显著(P<0.05)。对照组打斗次数与10-7 mol组差异不显著(P>0.05),与其余各组差异显著(P<0.05)。注射组中,随注射量的增加,打斗次数下降,10^-8 mol组与其余各组差异显著(P<0.05),10-7 mol组与10-6 mol组差异不显著(P>0.05)。打斗结束后,胜利方体内多巴胺含量与正常水平差异不显著(P>0.05),但显著高于失败方(P<0.05)。试验结果表明,注射多巴胺极显著影响中华绒螯蟹打斗行为(P<0.01),随多巴胺注射量的升高,中华绒螯蟹打斗行为整体下降。
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| 关 键 词： | 中华绒螯蟹 多巴胺 打斗行为 外源注射 |
Effect of Dopamine Injection on Aggressive Behavior of Chinese Mitten Crab |
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| DAI Liang,LI Xiaodong,LI Yi,LIU Handi,CAO Meng,ZHENG Yan,SUN Na.Effect of Dopamine Injection on Aggressive Behavior of Chinese Mitten Crab[J].Fisheries Science,2020,39(3):324-331. |
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| Authors: | DAI Liang LI Xiaodong LI Yi LIU Handi CAO Meng ZHENG Yan SUN Na |
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| Institution: | (Dalian Ocean University, Dalian 116023, China;Research & Development Center of Panjin Guanghe, Liaoning Key Laboratory of Chinese Mitten Crab Breeding, Professional and Technical Innovation Platform of Chinese Mitten Crab of Liaoning Guanghe, Enterprise Engineering Technology Research Center, Panjin 124200, China;Shenyang Agricultural University, Shenyang 110866, China) |
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| Abstract: | A total of 100 male first-year-old Chinese mitten crab Eriocheir sinensis in the same family were divided into four groups,and injected with 20μL saline(control group),10^-8,10-7 and 10-6 mol of dopamine solution at the arterial membranes of the fifth pereiopod.The crab was put into PE pipe with diameter of 20 cm and height of 40 cm(12 holes with diameter of 5 mm were drilled uniformly at the bottom).Two individuals were dumped in two opaque beakers in the test containers and placed on the opposite side of PE tube for 1 hour,and at the beginning of the experiment,two beakers were taken out at the same time.Closed-circuit camera was used to observe aggressive behavior,and to record the fighting duration,fighting frequency,fighting score and the relationship between success and failure of the two crabs within 4 hours to explore the effect of neurotransmitter dopamine on the aggressive behavior of Chinese mitten crab.At the end of the fight,test crabs and three crabs in the control group were quickly sampled,and the content of dopamine was determined by ELISA enzyme immunoassay in the hemolymph.It was found that there was the longest period of fighting in the crab in the control group,significant difference between the control group and the dopamine injection groups(P<0.05).In the dopamine injection groups,the fighting duration was decreased with the increase in the dopamine dose.The fighting intensity of crab was shown to be lower in control group than that in 10^-8 mol group,higher than those in other dopamine injection groups,and with significant difference between each injection group(P<0.05).In the dopamine injection groups,the fighting intensity was decreased with the increase in dopamine dosage,with the maximal fighting intensity in 10^-8 mol group,significant difference among the groups(P<0.05).There was no significant difference in fighting frequency between control group and 10-7 mol group(P>0.05),significant difference between control group and other groups(P<0.05).In the dopamine injection groups,fighting frequency was decreased with the increase in injection dosage.There was significant difference between 10^-8 mol group and other groups(P<0.05),without significant difference between 10-7 mol group and 10-6 mol group(P>0.05).At the end of the fighting,the winners had the same dopamine level in hemolymph as the normal level,significantly higher than those in the losers(P<0.05).The findings show that dopamine injection leads to significant decrease in fighting behavior of Chinese mitten crab as increase in dopamine dosage. |
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| Keywords: | Eriocheir sinensis dopamine aggressive behavior exogenous injection |
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