| 小花棘豆总生物碱抑菌作用的研究 |
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| 引用本文： | 王帅,李怀志,马明,张玲,马春晖.小花棘豆总生物碱抑菌作用的研究[J].新疆农业科学,2012,49(1):175-180. |
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| 作者姓名： | 王帅 李怀志 马明 张玲 马春晖 |
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| 作者单位： | 1. 新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300;塔里木大学动物科学学院,新疆阿拉尔843300
2. 新疆呼图壁种牛场,新疆呼图壁,831100 |
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| 基金项目： | 新疆生产建设兵团博士基金专项(2008JC07) |
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| 摘 要： | 【目的】研究小花棘豆总生物碱的抑菌作用。【方法】通过体外抑菌试验研究小花棘豆总生物碱的抑菌活性、最小抑菌浓度(MIC)、最小杀菌浓度(MBC)和影响小花棘豆总生物碱抑菌效果的因素。【结果】小花棘豆总生物碱对金黄色葡萄球菌、乳房链球菌、化脓链球菌和枯草芽孢杆菌有抑菌作用;其对乳房链球菌和化脓链球菌的MIC为6.3 mg/mL,对金黄色葡萄球菌的MIC为1.6 mg/mL,对枯草芽孢杆菌的MIC为0.8 mg/mL。生物碱在不同pH值下的抑菌活性发生变化;高温使其抑菌活性消失;紫外照射对其稳定性没有影响。【结论】小花棘豆总生物碱对部分革兰氏阳性菌有较好的抑菌作用。
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| 关 键 词： | 小花棘豆 生物碱 抑菌 |
Study on Bacteriostasis Efficiency of Total Alkaloids in Oxytropis glabra DC |
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| WANG Shuai
,
Li Huai-zhi
,
Ma Ming
,
ZHANG Ling
,
MA Chun-hui.Study on Bacteriostasis Efficiency of Total Alkaloids in Oxytropis glabra DC[J].Xinjiang Agricultural Sciences,2012,49(1):175-180. |
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| Authors: | WANG Shuai Li Huai-zhi Ma Ming ZHANG Ling MA Chun-hui |
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| Institution: | 1,2(1.Key laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production & Construction Corps,Alar Xinjiang 843300,China;2.College of Animal Science,Tarim University,Alar Xinjiang 843300,China;3.Hutubi County Cattle Farm,Hutubi Xinjiang 831100,China) |
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| Abstract: | 【Objective】The purpose of this project aim to study the antimicrobial activities of total alkaloids for Oxytropis glabra DC.【Method】Through bacteriostasis experiments in vitro,antimicrobial activities of total alkaloids for Oxytropis glabra DC on bacteriostasis efficiency,minimal inhibitory concentration,minimal bactericidal concentration,and environmental effect were studied.【Result】The result showed that alkaloid of Oxytropis glabra DC had fairly bacteriostasis for Staphylococcus aureus,Streptococcus uberis,Streptococcus pyogene,Bacillus subtilis;the MIC for Streptococcus uberis and Streptococcus pyogene was 6.3 mg/mL,the MIC for Staphylococcus aureus was 1.6 mg/mL,the MIC for Bacillus subtilis was 0.8 mg/mL.The diversity of bacteriostasis efficiency of alkaloid happened in different power of hydrogen;the bacteriostasis material has bad heat stability;ultraviolet ray treatment does not affect its stability.【Conclusion】 Total alkaloids of Oxytropis glabra DC had obvious bacteriostasis effect for Gram-positive bacteria. |
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| Keywords: | Oxytropis glabra DC alkaloid bacteriostasis |
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