| 紫外线诱变选育高产醋酸的醋酸菌研究 |
| |
| 引用本文： | 张蕾,郭龙,傅力.紫外线诱变选育高产醋酸的醋酸菌研究[J].新疆农业科学,2012,49(6):1120-1126. |
| |
| 作者姓名： | 张蕾 郭龙 傅力 |
| |
| 作者单位： | 新疆农业大学食品科学与药学学院,乌鲁木齐,830052;韩山师范学院生物系,广东潮州,521041 |
| |
| 基金项目： | 新疆维吾尔自治区科技计划项目(200931101-5);国家“十二五”科技支撑计划项目(2011BAD27B02-04) |
| |
| 摘 要： | 目的]选育适合酿造杏渣醋的高产醋酸的醋酸菌株.方法]对初始醋酸菌C1-0的生长曲线进行测定,对处于对数期的C1-0进行紫外线诱变,使诱变菌株的致死率达85;～90;,以产酸量为指标,选育高产醋酸的醋酸菌菌株,通过初筛和复筛,选育高产醋酸的醋酸菌菌株,并将其进行传代培养,测定产酸的稳定性.结果]采用紫外线对培养至24h的初始菌株C1-0照射20s,使其致死率达到88.72;,获得了3株高产醋酸的醋酸菌突变株C1-11、C1-27、C1-30,产酸量分别达到37.29、38.80和36.60g/L.这3株突变株传代5次,产酸量变化幅度较小.结论]通过紫外线诱变的方法获得三株高产醋酸的醋酸菌株C1-11、C1-27、C1-30,比初始菌株C1-0产酸量分别提高4.5;、8.6;、2.6;,且遗传稳定性较好.
|
| 关 键 词： | 紫外线诱变 选育 醋酸菌 突变株 |
| 收稿时间： | 2012-06-25 |
Study on Breeding of Mutants of High-yield Acetic Acid bacteria by Ultraviolet Radiation |
| |
| ZHANG Lei
,
GUO Long
,
FU Li.Study on Breeding of Mutants of High-yield Acetic Acid bacteria by Ultraviolet Radiation[J].Xinjiang Agricultural Sciences,2012,49(6):1120-1126. |
| |
| Authors: | ZHANG Lei GUO Long FU Li |
| |
| Institution: | 1.College of Food Science and Pharmaceutical Science,XinJiang Agricultural University,Urumqi 830052,China;2.Department of Biology,Hanshan Normal University,Chaozhou Guangzhou 521041,China) |
| |
| Abstract: | 【Objective】The purpose of this study was to breed acetic acid bacteria with high-yield acetic acid for brewing vinegar from apricot dregs.【Method】The growth curve of acetic acid bacteria C1-0 was determined,then the strain of C1-0 was mutated by ultraviolet irradiation.And the lethal rate of mutants was set 85%-90%,The high-yield strains of acetic acid bacteria were screened.By means of the experiments of determining both pH value and acid content,the acid production for 5 generations of mutants was measured.【Result】 The strain of C1-0 was irradiated for 20s by UV when it came into logarithmic growth phase after it was incubated for 24 h,and,the lethal rate was 88.72%.Three high-yield mutants of C1-11,C1-27 and C1-30 have been obtained.The acid yield of C1-11,C1-27 and C1-30 were 37.29,38.80 and 36.60 g/L respectively.The acid yield for 5 generations of mutants changed little.【Conclution】The mutants of C1-11,C1-27,C1-30 with high-yield acetic acid by ultraviolet radiation were obtained,the acid yield of C1-11,C1-27,C1-30 were increased by 4.5%,8.6%,2.6% than C1-0,and genetic stability was good. |
| |
| Keywords: | ultraviolet radiation breeding acetic acid bacteria mutant |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《新疆农业科学》浏览原始摘要信息 |
| 点击此处可从《新疆农业科学》下载免费的PDF全文 |
|
