| 一株棉秆纤维素分解真菌的分离筛选及酶学性质研究 |
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| 引用本文： | 白宝伟,张琴,滕立平,杨瑛,李艳宾.一株棉秆纤维素分解真菌的分离筛选及酶学性质研究[J].新疆农业科学,2012,49(4):701-707. |
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| 作者姓名： | 白宝伟 张琴 滕立平 杨瑛 李艳宾 |
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| 作者单位： | 塔里木大学生命科学学院,新疆阿拉尔,843300;塔里木大学植物科学学院,新疆阿拉尔,843300;塔里木大学机械电气化工程学院,新疆阿拉尔,843300 |
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| 基金项目： | 国家自然科学基金(30960071) |
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| 摘 要： | 目的]对从棉秆腐解物中筛选得到的高效棉秆分解真菌SJ-1进行纤维素酶酶学性质的研究.方法]研究温度、pH、金属离子对纤维素酶活力的影响,并以Lineweaver -Burk作图法测定酶促反应米氏常数Km及最大反应速率Vmax.结果]该菌株CMCase和FPase的最适反应温度在50～60℃,最适pH为7.0,有较好的耐高温及耐碱能力.K+、Fe2对酶活有显著的激活作用,而Cu2+、Mg2+、Ca2+、Al3+等有一定的抑制作用,其中Al3的抑制作用较为强烈,Cu2+对FPase抑制较强,Mn2-对FPase有激活作用而对CMCase有抑制作用,Zn2对酶活性无明显影响.以CMC - Na做底物时酶反应的Km为2.69 mg/mL,Vmax为0.53 mg/( mL·min).结论]菌株SJ-1的纤维素酶性质较为优良,为进一步进行菌株的选育与改造提供参考依据.
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| 关 键 词： | 棉秆 腐解 纤维素分解菌 纤维素酶 酶学性质 |
| 收稿时间： | 2012-04-25 |
Isolation of a Cotton Stalk Cellulose Decomposing Fungus and Its cellulase Properties |
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| BAI Bao-wei
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ZHANG Qin
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TENG Li-ping
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YANG Ying
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LI Yan-bin.Isolation of a Cotton Stalk Cellulose Decomposing Fungus and Its cellulase Properties[J].Xinjiang Agricultural Sciences,2012,49(4):701-707. |
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| Authors: | BAI Bao-wei ZHANG Qin TENG Li-ping YANG Ying LI Yan-bin |
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| Institution: | 1(1.College of Life Science,Tarim University,Alaer Xinjiang 843300,China;2.College of Plant Science,Tarim University,Alaer Xinjiang 843300,China;3.College of Mechanical and Electronic Engineering,Tarim University,Alaer Xinjiang 843300,China) |
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| Abstract: | 【Objective】The purpose of this project was to study cellulase pr operties of a cellulose decomposing f ungus SJ-1 isolated from cotton stalk decomposing products were studied.【Method】 Effects of temperature,pH value and metal ions on cellulase activity were s tudied,and Michaelis constant Km of enzymatic reaction and the maximum reaction rate Vmax were examined by Lineweaver-Burk method.【Result】The best reaction temperature to CMCase and FPase was ranged from 50 to 60℃,and the best pH va lue was 7.0,which showed the tolerance to high temperature and alkali.K+ and Fe 2+ activated the cellulase activity significantly,while Cu2+,Mg2+,Ca 2+ and Al3+ showed certain inhibition,the inhibition of Al3+ exhibited much more stro ngly, Cu2+ inhibited FPase strongly as well,Mn2+ showed activation to FPase but inhi bition to CMCase,Zn2+ had no influence on cellulase activity.When CMC-Na was t aken as s ubstra te,Km and Vmax of the enzymatic reaction were 2.69 mg/mL and 0.53 mg/(mL·min) respectively.【Conclusion】The strain showed good properties of cellulase activ ity,and provided references for further breeding and alteration of this fungus. |
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| Keywords: | cotton stalk decomposition cellulose decomposing microorganism cellulase enzymatic properties |
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