| 牦牛源多杀性巴氏杆菌ompH基因原核表达及抗原性鉴定 |
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| 引用本文： | 田亮,康立超,赵文娟,薄新文,马勋.牦牛源多杀性巴氏杆菌ompH基因原核表达及抗原性鉴定[J].新疆农业科学,2012,49(1):146-149. |
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| 作者姓名： | 田亮 康立超 赵文娟 薄新文 马勋 |
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| 作者单位： | 石河子大学,新疆石河子832003;新疆农垦科学院新疆兵团绵羊繁育生物技术重点实验室,新疆石河子832000;新疆农垦科学院新疆兵团绵羊繁育生物技术重点实验室,新疆石河子,832000;石河子大学,新疆石河子,832003 |
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| 基金项目： | 新疆农垦科学院科技引导计划(YYD201107);新疆农垦科学院青年基金(YQJ2009-02,YQJ201110) |
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| 摘 要： | 目的]克隆、表达牦牛源多杀性巴氏杆菌(Pm)外膜蛋白基因H(ompH)并鉴定其抗原性.方法]扩增牦牛源多杀性巴氏杆菌去信号肽的ompH基因,构建原核表达载体pET28a - ompH,转化大肠杆菌BL21( DE3)并诱导表达,通过SDS-PAGE鉴定表达目的蛋白,利用Western blot检测该蛋白的抗原性.结果]成功克隆、构建了牦牛源多杀性巴氏杆菌去信号肽的pET28a - ompH原核表达载体.诱导表达蛋白约38 kDa,Western blot检测重组蛋白具有良好的抗原性.结论]ompH基因的成功表达为重组OmpH蛋白的血清学检测方法的建立、多克隆抗体的制备及疫苗的研制奠定了基础.
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| 关 键 词： | 牦牛 多杀性巴氏杆菌 ompH基因 原核表达 |
| 收稿时间： | 2012-01-25 |
Expression and Antigenicity Analysis of the OmpH Gene of Pasteurella multocidafrom Yak |
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| TIAN Liang
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KANG Li-chao
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ZHAO Wen-juan
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BO Xin-wen
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MA Xun.Expression and Antigenicity Analysis of the OmpH Gene of Pasteurella multocidafrom Yak[J].Xinjiang Agricultural Sciences,2012,49(1):146-149. |
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| Authors: | TIAN Liang KANG Li-chao ZHAO Wen-juan BO Xin-wen MA Xun |
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| Institution: | 1(1.College of Animal Science & Technology,Shihezi University,Shihezi Xinjiang 832003,China;2.Xinjiang Academy of Agricultural and Reclamation Sciences/Key Laboratory of Sheep Breeding Biology Technology of Xinjiang Production and Construction Corps,Shihezi Xinjiang 832000,China) |
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| Abstract: | 【Objective】The study aims to clone and express the recombinant OmpH preotein of Pasteurella multocida(Pm) from yak.【Methods】The deleted signal peptide OmpH fragment of Pm from yak was amplified.The prokaryotic expression plasmid of pET28a-ompH was constructed.It was transformed into BL21(DE3) and was expressed.SDS-PAGE was carried out to identify the protein,and Western blot was employed to determine the reaction of the target protein with the Pm antibody.【Result】The construction of the prokaryotic expression plasmid of pET28a-ompH was successful.The expressed protein was about 38kDa and reactive in the Western blot detection.【Conclusion】The successful expression of OmpH gene takes a shot at further serological detection,polyclonal antibody preparation and vaccine development. |
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| Keywords: | yak Pasteurellosis multocida ompH gene expression |
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