| 小鼠卵母细胞孤雌激活试验 |
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| 引用本文： | 武浩,步同章,屈鹏辉,张涌.小鼠卵母细胞孤雌激活试验[J].畜牧兽医杂志,2004,23(2):1-3. |
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| 作者姓名： | 武浩 步同章 屈鹏辉 张涌 |
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| 作者单位： | 西北农林科技大学动物科技学院,陕西,杨凌,712100 |
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| 摘 要： | 应用两种激活方法(乙醇和离子霉素 6-DMAP)激活和三种培养液(CZB，KSOM，M199)对90只小鼠卵母细胞进行了孤雌激活研究。结果显示，离子霉素结合6-DMAP的激活效果高于乙醇；三种培养液中，以CZB激活效果较为理想。在随后的卵母细胞发育率方面，CZB、KSOM和M199三者之间的差异极为明显，以CZB最高，KSOM次之，M199的发育率最低。
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| 关 键 词： | 小鼠 卵母细胞 孤雌激活 激活条件 培养液 体外发育 |
| 文章编号： | 1004-6704(2004)02-0001-03 |
| 修稿时间： | 2003年11月1日 |
Parthenogenetic activation on mouse oocytes |
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| WU Hao,BU Tong-zhang,QU Peng-hui,ZHGANG Yong.Parthenogenetic activation on mouse oocytes[J].Journal of Animal Science and Veterinary Medicine,2004,23(2):1-3. |
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| Authors: | WU Hao BU Tong-zhang QU Peng-hui ZHGANG Yong |
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| Abstract: | Two activation methods(alcohol and ionomycin 6-DMAP) and three culture mediums(CZB,KSOM and M199) were used for study of oocyte parthenogenetic activation in 90 mice.The result showed: The activation effect of ionomycin 6-DMAP was better than alcohol; Among three mediums, CZB was the best on parthenogenetic activation of mouse oocytes. There had a significant difference among CZB,KSOM and M199 at the development rate of oocytes,in proper order was CZB,KSOM and M199. |
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| Keywords: | oocyte parthenogenetic activation development rate mouse |
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