| 禾谷镰孢漆酶样多铜氧化酶的鉴定及其表达模式 |
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| 引用本文： | 刘宁,渠清,李丽娜,庞茜,柳建虎,张垚,曹志艳,董金皋.禾谷镰孢漆酶样多铜氧化酶的鉴定及其表达模式[J].植物病理学报,1955,49(6):763-772. |
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| 作者姓名： | 刘宁 渠清 李丽娜 庞茜 柳建虎 张垚 曹志艳 董金皋 |
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| 作者单位： | 河北省植物生理与分子病理学重点实验室,河北农业大学真菌毒素与植物分子病理学实验室,保定 071000 |
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| 基金项目： | 现代农业产业技术体系专项(CARS-02-25);国家重点研发计划—粮食丰产增效科技创新专项(2016YFD0300704) |
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| 摘 要： | 子囊菌禾谷镰孢(Fusarium graminearum)可侵染玉米茎部造成严重的玉米茎腐病。漆酶样多铜氧化酶(Laccase-like multicopper oxidase)具有广泛的作用底物且在植物病原真菌中参与病菌侵染,促进病菌定殖。利用已知真菌漆酶的蛋白序列在禾谷镰孢中鉴定得到14个漆酶样多铜氧化酶,分属5种亚家族。通过对其在侵染玉米茎部不同时间后的芯片数据分析表明FGSG_02142、FGSG_05159在菌丝、孢子及侵染各阶段表达量均较高,FGSG_02328、FGSG_13185和FGSG_00142在侵染阶段表达量明显增加,其他基因表达量相对较低;试验进而利用qPCR检测了部分差异基因在接种玉米感病种质资源B73和抗病种质资源Mo17中的相对表达量,结果显示,禾谷镰孢FGSG_00142基因在B73中的表达量明显高于在抗病材料Mo17中的表达量,而毒素相关基因FGSG_02328在接种Mo17时表达也出现延迟,推测这2个基因均参与了禾谷镰孢的侵染过程。
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| 关 键 词： | 禾谷镰孢 漆酶 玉米茎腐病 表达模式 |
Identification of laccase-like multicopper oxidases in Fusarium graminearum and expression profiling during maize stalk infection |
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| LIU Ning,QU Qing,LI Li-na,PANG Xi,LIU Jian-hu,ZHANG Yao,CAO Zhi-yan,DONG Jin-gao.Identification of laccase-like multicopper oxidases in Fusarium graminearum and expression profiling during maize stalk infection[J].Acta Phytopathologica Sinica,1955,49(6):763-772. |
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| Authors: | LIU Ning QU Qing LI Li-na PANG Xi LIU Jian-hu ZHANG Yao CAO Zhi-yan DONG Jin-gao |
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| Institution: | Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Mycotoxin and Molecular Plant Pathology Laboratory of Hebei Agricultural University, Baoding 071000, China |
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| Abstract: | Fusarium graminearum, the pathogenic fungus of maize stalk rot, can infect the stalk of maize and invade plant cells. Laccase-like multicopper oxidase is a multicopper oxidase with a wide range of substrates and play important physiological roles during growth and pathogenesis of the fungus. Fourteen laccase-like multicopper oxidases were identified in the genome of F. graminearum by comparison with the protein sequence of fungal laccases, which belong to five superfamilies. Using gene chip data of stalk infection during 12-144 h, the expression of these genes was analyzed. The expressions of FGSG_02142 and FGSG_05159 in the mycelium, spore and infection stages were at a high level. The expression of FGSG_02328, FGSG_13185 and FGSG_00142 increased during the infection stage. The expression of other genes was relatively low. qPCR was used to analyze the laccase gene family in F. graminearum inoculated susceptible cultivar B73 and resistant cultivar Mo17. It was observed that the expression level of FGSG_00142 was higher in fungus-inoculated susceptible cultivar B73 than resistant cultivar Mo17. Moreover, the expression of FGSG_02328 which participated in the toxin biosynthesis was delayed when inoculated to resistant cultivar Mo17. FGSG_00142 and FGSG_02328 might play roles in infection of F. graminearum. |
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| Keywords: | Fusarium graminearum laccase maize stalk rot expression profiling |
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