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| 甘薯杆状病毒湖南分离物基因组全序列测定及比较分析 | |
| 引用本文： | 黄艳岚,张超凡,邹学校.甘薯杆状病毒湖南分离物基因组全序列测定及比较分析[J].植物病理学报,1955,49(6):790-798. |
| 作者姓名： | 黄艳岚 张超凡 邹学校 |
| 作者单位： | 湖南农业大学园艺园林学院, 长沙410128; 湖南省作物研究所, 长沙410125; 湖南农业大学, 长沙410128 |
| 基金项目： | 湖南省农业科技创新团队(2017XC13);湖南省自然科学基金(2016JJ6070);湖南省甘薯工程技术研究中心(2018TP2032);国家现代农业产业技术体系(CARS-11-C-16) |
| 摘 要： | 利用Small RNA测序技术对采自湖南的甘薯杆状病毒进行鉴定,获得与SPBV-B序列具有相似性的contigs 161个。首先设计小片段引物,PCR扩增分离出706 bp的目的片段,与SPBV-B (FJ560944)相似性为78%。分段设计引物,进行基因全序列PCR扩增。引物组合SPBV-BF1/R1、SPBV-BF2/R4和SPBV-BF5/R5分别扩增出3 150、2 900和3 500 bp目的条带。序列拼接获得2个SPBV-B基因组全序列,大小分别为7 894 bp(MK052980)和7 981 bp(MK052981),包含完整编码阅读框。进化分析表明MK052980和MK052981与SPPV聚为同一分支,与SPPV相似性分别为81%和83%,与SPBV-B相似性分别为86%和94%。MK052980和MK052981具有89.5%的相似性。经编码阅读框氨基酸序列比对,MK052980序列的ORF3a氨基酸序列存在变异。这是国内首次报道SPBV-B全基因序列。 |
| 关 键 词： | Small RNA测序 杆状病毒 甘薯杆状病毒 全基因序列 序列分析 |
Complete genome sequencing and comparative analysis of Sweet potato badna-virus isolates from Hunan |
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| HUANG Yan-lan,ZHANG Chao-fan,ZOU Xue-xiao.Complete genome sequencing and comparative analysis of Sweet potato badna-virus isolates from Hunan[J].Acta Phytopathologica Sinica,1955,49(6):790-798. | |
| Authors: | HUANG Yan-lan ZHANG Chao-fan ZOU Xue-xiao |
| Institution: | College of Horticulture, Hunan Agricultural University, Changsha410128,China; Crop Research Institute of Hunan, Changsha410125,China; Hunan Agricultural University, Changsha410128,China |
| Abstract: | Sweet potato badnavirus, collected from Hunan province, were identified by Small RNA sequencing, and got 161 contigs homologous with the SPBV-B sequence. The target fragment of 706 bp was amplified by PCR ,which was 78% homologous to SPBV-B(FJ560944). Three fragments of Sweet potato badnavirus B sequence, whose length were 3 150 bp,2 900 bp and 3 500 bp, were amplified by SPBV-BF1/R1, SPBV-BF2/R4 and SPBV-BF5/R5 primers, respectively.Two complete genomic sequences of SPBV-B,7 894 bp(MK052980) and 7 981 bp (MK052981) , obtained by sequence assembly, contained complete ORFs. Phylogenetic analysis showed that MK052980,MK052981 and SPPV were in the same group with 81% and 83% sequencing similarity, respectively,and shared 86% and 94% homologies with SPBV-B, respectively. MK052980 and MK052981 were 89.5% sequence similarity. The ORF3a amino acid sequences of MK052980 was mutated by sequence alignment. It′s the first report the complete genome sequences of SPBV-B in China. |
| Keywords: | Small RNA sequencing badnavirus SPPV complete genome sequence sequence analysis |
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