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陕西省猕猴桃黑斑病病原菌鉴定
引用本文:付博,王家哲,任平,李英梅,赵杰,金平涛,张锋.陕西省猕猴桃黑斑病病原菌鉴定[J].植物病理学报,1955,50(1):112-116.
作者姓名:付博  王家哲  任平  李英梅  赵杰  金平涛  张锋
作者单位:陕西省生物农业研究所 陕西省植物线虫学重点实验室,西安 710043;
陕西省酶工程技术研究中心,西安 710600;
西北农林科技大学植物保护学院,杨凌 712100;
陕西省周至县植保植检站,周至 710400
基金项目:陕西省科学院优秀青年科研项目暨青年联合创新项目(2016K-19); 西安市农业主导产业发展项目(201806116YF04NC12); 陕西省重点研发计划项目(2018ZDXM-NY-067)
摘    要:


Identification of the pathogen causing black spot on kiwifruit in Shaanxi Province
FU Bo,WANG Jia-zhe,REN Ping,LI Ying-mei,ZHAO Jie,JIN Ping-tao,ZHANG Feng.Identification of the pathogen causing black spot on kiwifruit in Shaanxi Province[J].Acta Phytopathologica Sinica,1955,50(1):112-116.
Authors:FU Bo  WANG Jia-zhe  REN Ping  LI Ying-mei  ZHAO Jie  JIN Ping-tao  ZHANG Feng
Institution:Bio-Agriculture Institute of Shaanxi, Shaanxi Key Laboratory of Plant Nematology, Xi’an 710043, China;
Enzyme Engineering Research Center of Shaanxi Province, Xi’an 710600, China;
Northwest A&F University, College of Plant Protection, Yangling 712100, China;
Zhouzhi Plant Protection and Quarantine Station in Shaanxi Province, Zhouzhi 710400, China
Abstract:To confirm the causal pathogen and damage degree of a new disease of kiwifruit-black spot in Shaanxi Province, a field investigation was conducted in Zhouzhi County and Huyi District in 2018. The pathogen was isolated from the diseased fruits and identified based on morphological characteristics, pathogenicity testing, sequence alignment and phylogenic tree analysis. The results showed that the average incidence of black spot was 65%, and the early fruiting rate was 33%. Fruits infected with black spot disease were soft-rotted after being stored for two months under low temperature conditions. The pathogenic fungi were isolated from the under skin of Cuixiang kiwifruits and the strains could produce two types of conidia, including oval α-type conidia and curved β-type conidia. Through sequences alignment of 18S rRNA, ITS, reference gene (EF1-α) and phylogenetic tree clustering analysis, it was identified for the first time that the causal pathogen causing black spot disease of Cuixiang kiwifruit in Shaanxi Province was Diaporthe eres.
Keywords:Kiwifruit  black spot  pathogenic fungi  Diaporthe eres  
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