| 江苏省小麦苗枯病菌的遗传多样性初析 |
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| 引用本文： | 尹燕妮,张晓梅,葛芸英,郭坚华.江苏省小麦苗枯病菌的遗传多样性初析[J].南京农业大学学报,2006,29(1):44-48. |
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| 作者姓名： | 尹燕妮 张晓梅 葛芸英 郭坚华 |
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| 作者单位： | 南京农业大学农业部病虫监测与治理重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 中国科学院资助项目;国家科技攻关项目 |
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| 摘 要： | 采用ERIC-PCR、BOX-PCR和ITS技术，对江苏省6个市的24个小麦苗枯病菌（Clavibacter fangii）进行遗传多样性分析，并与其他10种病原细菌进行比较。结果表明，在相似率达60％时，ITS将24个小麦苗枯病菌分成了5簇。以相似性60％为界，ERIC-PCR将34个参试菌株分为14簇，而BOX．PCR只得到9簇，暗示这两种短重复序列在基因组中的分布不同；将两者电泳图谱结合，得到介于上述两者问的结果，所有菌株被分成12簇，24个小麦苗枯病菌分布在5簇中。3种分析方法相互验证，均说明江苏省小麦苗枯病菌基因组存在显著多样性。ERIC-PCR和BOX-PCR聚类证明了小麦苗枯病菌与棒形杆菌属（Clavibacter）亲缘关系较近，与其他属细菌亲缘关系较远。ERIC-PCR和BOX-PCR扩增基因组DNA指纹比ITS图谱具有更强的多样性。
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| 关 键 词： | 小麦苗枯病菌 遗传多样性 |
| 文章编号： | 1000-2030(2006)01-0044-05 |
| 收稿时间： | 05 9 2005 12:00AM |
| 修稿时间： | 2005年5月9日 |
Genetic diversity of wheat pathogen Clavibacter fangii from Jiangsu Province, China |
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| YIN Yan-ni,ZHANG Xiao-mei,GE Yun-ying,GUO Jian-hua.Genetic diversity of wheat pathogen Clavibacter fangii from Jiangsu Province, China[J].Journal of Nanjing Agricultural University,2006,29(1):44-48. |
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| Authors: | YIN Yan-ni ZHANG Xiao-mei GE Yun-ying GUO Jian-hua |
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| Institution: | Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Twenty-four Clavibacter fangii strains collected from six cities of Jiangsu Province and ten other phytopathogenic bacteria were analysed according to genotypic typing methods including BOX-PCR,ERIC-PCR,and ITS profiling(PCR ribotyping).The analysis of ITS gene gave 5 clusters of Cl.fangii at the level of 60% similarity after dendrogram analysis.ERIC-PCR revealed that there were 14 clusters in 34 strains,while with BOX-PCR method only nine(maximum similarity approx 60%).At the level of 60% similarity,the combination of ERIC-PCR and BOX-PCR profiles gave 12 clusters of 34 strains,24 Cl.fangii strains among which were divided into 5 clusters.In summary,the results of ERIC-PCR and BOX-PCR dendrogram analysis suggest that Cl.fangii is only genetically close related to genus Clavibacter,and that ERIC-PCR and BOX-PCR are higher discriminatory techniques than ITS profiling for detecting genetic variation among strains and for identification as well as classification studies. |
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| Keywords: | rep-PCR ITS |
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