| 酸奶中传统发酵乳酸菌的分离筛选及增菌研究 |
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| 引用本文： | 侯小歌,郭翠红,金刚,许静.酸奶中传统发酵乳酸菌的分离筛选及增菌研究[J].安徽农业科学,2009,37(13):5854-5856. |
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| 作者姓名： | 侯小歌 郭翠红 金刚 许静 |
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| 作者单位： | 周口师范学院生命科学系,河南周口,466001;周口师范学院生命科学系,河南周口,466001;周口师范学院生命科学系,河南周口,466001;周口师范学院生命科学系,河南周口,466001 |
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| 基金项目： | 河南省周口师范学院青年科研基金资助项目(zknuqn2008-34) |
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| 摘 要： | 目的]分离筛选传统发酵乳酸菌，选择最适增菌物质，方法]以市售酸奶为试材，采用稀释涂布法和平板划线法分离纯化保加利亚乳杆菌（Lactobacillus bulgaricus）和嗜热链球菌（Streptoeocus thermophilus），分别采用4种培养基分离，通过菌落观察、镜检和理化特性测定，筛选优良菌株、在最适分离液态培养基中添加6种物质对菌株进行增菌培养，每隔8h测定菌液0D值。结果]L．bulgaricus和Str．thermophilus在改良培养基上生长良好，测定理化特性筛选出发酵性能优艮的2株，L.bulgnricus（L．b．2、L．b．3）和1株斯．thermophilus（S．t．3）。增菌结果显示5种天然营养物均不同程度地促进了三．bulgaricus和Str．thermophilus的生长.结论]改良MRS培养基和改良M17培养基分别为，L．bulzaricus和Str．thermophilus的最佳分离培养基；10％黄豆芽汁和12％胡萝卜汁是L．bulgaricus的最佳增菌物质。
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| 关 键 词： | 保加利亚乳杆菌 嗜热链球菌 分离筛选 增菌 |
Study on the Isolation,Screening and Promoting Bacteria of the Traditional Fermenting Lactic Acid Bacteria from Yoghourt |
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| HOU Xiao-ge et al.Study on the Isolation,Screening and Promoting Bacteria of the Traditional Fermenting Lactic Acid Bacteria from Yoghourt[J].Journal of Anhui Agricultural Sciences,2009,37(13):5854-5856. |
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| Authors: | HOU Xiao-ge |
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| Institution: | HOU Xiao-ge et al (College of Life Science,Zhoukou Normal University,Zhoukou,Henan 466001) |
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| Abstract: | Objective]The research aimed to isolate and screen out the traditional fermenting lactic acid bacteria and select the optimum bacteria-promoting materials.Method]Taking the yoghourt on sale as the tested materials,L.bulgaricus and Str.thermophilus were isolated and purified by using the diluted and streaked plate method.L.bulgaricus and Str.Thermophilus were isolated by using 4 kinds of media.Through the colony observation,microscopic examination and the determination of physiological and biochemical char... |
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| Keywords: | L bulgaricus Str thermophilus Isolation and screening Promoting medium |
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