| 苎麻纤维质酶降解生产生物燃料乙醇的工艺 |
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| 引用本文： | 彭源德,郑科,杨喜爱,严理,唐守伟,朱爱国,熊和平.苎麻纤维质酶降解生产生物燃料乙醇的工艺[J].农业工程学报,2007,23(4):6-10. |
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| 作者姓名： | 彭源德 郑科 杨喜爱 严理 唐守伟 朱爱国 熊和平 |
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| 作者单位： | 中国农业科学院麻类研究所,长沙,410205 |
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| 基金项目： | 引进国际先进农业科技计划(948计划);中国农业科学院基金;湖南省重点实验室基金 |
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| 摘 要： | 在微生物预处理苎麻韧皮的基础上，进行了pH值、酶用量、葡萄糖浓度和原料处理方式等对苎麻纤维质酶降解的单因子试验和正交试验。结果表明，苎麻纤维质酶解的起始pH值为5.0左右，总糖转化率最高，达72.361％；在一定的酶浓度范围内，随着酶量的增加，苎麻纤维质的总糖转化率提高，酶的适宜用量为：木聚糖酶5％、纤维素酶10％；水解液中葡萄糖浓度在0.5％以下时，苎麻纤维质的总糖转化率不受影响，葡萄糖浓度在0.5％以上时，随着水解液中葡萄糖含量的增加，总糖转化率下降；苎麻纤维质酶解过程中，对原料进行机械处理，有利于提高酶的水解效率；苎麻纤维质酶解的最适条件为：pH值4.5～5.5、纤维素酶浓度8%～11％、葡萄糖浓度小于0.5%、原料不洗直接进行剪碎处理。
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| 关 键 词： | 苎麻 纤维质 酶降解 影响因素 |
| 文章编号： | 1002-6819（2007）4-0006-05 |
| 收稿时间： | 2006/6/13 0:00:00 |
| 修稿时间： | 2006-06-132007-03-20 |
Technology for producing bio-ethanol from ramie lignoellulosic degradation with enzymes |
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| Peng Yuande,Zheng Ke,Yang Xi'ai,Yan Li,Tang Shouwei,Zhu Aiguo,Xiong Heping.Technology for producing bio-ethanol from ramie lignoellulosic degradation with enzymes[J].Transactions of the Chinese Society of Agricultural Engineering,2007,23(4):6-10. |
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| Authors: | Peng Yuande Zheng Ke Yang Xi'ai Yan Li Tang Shouwei Zhu Aiguo Xiong Heping |
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| Institution: | Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China |
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| Abstract: | After being pretreated on ramie bast fiber by microbial retting, both single factor and multi-factors were experimented during ramie lignoeellulosie degrading with enzymes, these influential factors are pH value, concentrations of enzymes and glucose, and methods for material treatment. The results show that, the highest rate of saeeharifieation reaches 72. 361% at the beginning of pH 5.0% Within a range of enzymes concentration, the rate of saecharification can be increased with the increase of enzymes ; The optimal enzyme concentrations are 5% of xylanase and 10% of eellulase, respectively; The rate of saeeharifieation is not influenced as glucose concentration in the solution of lignoeellulosie degrading is below 0.5 %, and as over 0.5 % of glucose concentration and with the increase of glucose in the solution, the rate of saeeharifieation is deereased; Mechanical treatment on material is beneficial for improving the efficiency of enzymatic degrading. The most optimum conditions for ramie enzymatic lignoeellulosie degrading is pH 4.5- 5.5, 8 0%- 11% of cellulose concentration, below 0.5 % of glucose concentration and treatment of seissorssed and unwatered material. |
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| Keywords: | ramie lignocellulose enzymatic degrading influential factors |
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