| 云南省玉溪市玉米致死性坏死病毒原的分子鉴定 |
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| 引用本文： | 李帅#,朱敏#,夏子豪,王廿,周涛,董家红,陈永对,张仲凯,范在丰.云南省玉溪市玉米致死性坏死病毒原的分子鉴定[J].植物保护,2015,41(3):110-114. |
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| 作者姓名： | 李帅# 朱敏# 夏子豪 王廿 周涛 董家红 陈永对 张仲凯 范在丰 |
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| 作者单位： | 1. 东北农业大学农学院, 哈尔滨150030; 2. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京100193 |
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| 基金项目： | 公益性行业（农业）科研专项（201103022） |
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| 摘 要： | 玉米致死性坏死病是由玉米褪绿斑驳病毒(Maize chlorotic mottle virus,MCMV)和一种或多种马铃薯Y病毒科病毒复合侵染引起的。2014年1月在对云南省玉溪市玉米病毒病害的调查中发现了一些表现严重花叶、矮化叶片甚至整个植株坏死症状的玉米。对采集样品进行RT-PCR检测,所有样品中都同时检测到了MCMV和甘蔗花叶病毒(Sugarcane mosaic virus,SCMV),在一个样品中同时检测到了MCMV、SCMV和南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus)。
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| 关 键 词： | 麦长管蚜 OBP7 RNA干扰 荧光定量PCR |
| 收稿时间： | 2014/3/25 0:00:00 |
| 修稿时间： | 2014/6/10 0:00:00 |
Molecular identification of the viruses causing maize lethal necrosis disease in Yuxi, Yunnan Province |
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| Li Shuai,Zhu Min,Xia Zihao,Wang Nian,Zhou Tao,Dong Jiahong,Chen Yongdui,Zhang Zhongkai,Fan Zaifeng.Molecular identification of the viruses causing maize lethal necrosis disease in Yuxi, Yunnan Province[J].Plant Protection,2015,41(3):110-114. |
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| Authors: | Li Shuai Zhu Min Xia Zihao Wang Nian Zhou Tao Dong Jiahong Chen Yongdui Zhang Zhongkai Fan Zaifeng |
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| Institution: | 1. College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | Maize lethal necrosis disease is caused by synergistic interaction between Maize chlorotic mottle virus (MCMV) and one or several potyviruses. Severe mosaic, leaf necrosis (even plant lethal necrosis) or rough dwarf were found during a survey on virus diseases in maize carried out in Yuxi District, Yunnan Province in January 2014. RT-PCR, molecular cloning and sequence analysis were performed to identify the viruses present in the sampled plants. The results showed that all of the diseased samples tested were co-infected with MCMV and Sugarcane mosaic virus (SCMV) and one of the plants was co-infected with MCMV, SCMV and Southern rice black-streaked dwarf virus (SRBSDV). |
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| Keywords: | Sitobion miscanthi odorant binding protein 7 RNA interference real-time PCR |
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