Binding of chlorpyrifos and cypermethrin to blood proteins |
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| Authors: | Yong Cui Jiangfeng Guo Ziyuan Chen |
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| Institution: | a Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China
b Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China |
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| Abstract: | The features of two insecticides (chlorpyrifos and cypermethrin) binding to two blood proteins, bovine serum albumin (BSA), and bovine hemoglobin (BHb), were investigated via the fluorescence method. The results revealed that both insecticides caused the fluorescence quenching of BSA and the fluorescence enhancement of BHb. A new parameter (FE), i.e., the fluorescence intensity when adequate insecticide was added, was introduced to obtain the association constant (KA) and the number of binding sites (n). KA and n of chlorpyrifos and cypermethrin binding to BSA were 2.99 × 105 and 5.22 × 105 L mol−1, 1.25 and 0.78, respectively. KA and n of chlorpyrifos and cypermethrin binding to BHb were 2.94 × 104 and 2.48 × 104 L mol−1, 1.75 and 2.19, respectively. In conclusion, chlorpyrifos and cypermethrin could bind to BSA and BHb, and the binding of both insecticides to BSA was significantly stronger than that of insecticides to BHb. These could affect the distribution, metabolism, and excretion of insecticides. |
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| Keywords: | Chlorpyrifos Cypermethrin Albumin Hemoglobin Fluorescence |
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