Selection and characterization of spinosad resistance in Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) |
| |
| Authors: | Wei Wang Jia’an Cheng Zhenhua Tang |
| |
| Institution: | a Institute of Applied Entomology, Zhejiang University at Huajiachi, No. 268, Kaixuan Road, Hangzhou, Zhejiang 310029, China
b Shanghai Institute of Plant Physiology and Ecology, Academia Sinica, No. 300, Fenglin Road, Shanghai 200032, China |
| |
| Abstract: | The cross-resistance and biochemical mechanism of the beet armyworm, Spodoptera exigua (Hübner), to spinosad was studied in the laboratory. S. exigua population were collected from Shanghai suburb. After five generations of selection, the resistance of S. exigua to spinosad increased 345.4 times compared with the susceptible strain. There was no cross-resistance between spinosad and fenvalerate, phoxim, methomyl, abamectin, and cyfluthrin. When the inhibitors, PBO, TPP, DEF, and DEM were used as synergist in the susceptible strain and resistant strain, the synergistic ratio was 0.7-, 0.5-, 1.0-, and 0.6- fold for the susceptible strain, and 9.8-, 1.5-, 2.6-, and 1.5-fold for the resistant strain, respectively. The results revealed that PBO had significant synergistic effect on the resistant strain. The activity in vitro of microsomal-O-demethylase and glutathione S-transferase in the resistant strain was 5.2- and 1.0-fold of the susceptible strain, respectively. The results implied that microsomal-O-demethylase might be important in conferring spinosad resistance in the S. exigua population. |
| |
| Keywords: | Spodoptera exigua Spinosad Synergism Cross-resistance |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
