| 甘肃河西马铃薯根际生防木霉菌对接骨木镰刀菌的拮抗筛选及鉴定 |
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| 引用本文： | 张茹,李金花,柴兆祥,王蒂.甘肃河西马铃薯根际生防木霉菌对接骨木镰刀菌的拮抗筛选及鉴定[J].草业学报,2009,18(2):138-145. |
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| 作者姓名： | 张茹 李金花 柴兆祥 王蒂 |
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| 作者单位： | 1.甘肃省作物遗传与种质创新重点实验室,甘肃 兰州 730070;2.甘肃农业大学农学院,甘肃 兰州 730070;3.甘肃农业大学草业学院,甘肃 兰州 730070 |
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| 摘 要： | 从甘肃省西部地区农牧交错区的马铃薯根际土壤中共分离到108株木霉菌株,通过平板对峙法筛选出10株对马铃薯干腐病主要病菌接骨木镰刀菌拮抗效果较好的木霉菌株,抗性系数均在0.826以上,其中天祝3株、永登4株、山丹3株。根据形态学特征、培养性状和ITS序列分析,将这10株生防木霉菌鉴定为哈茨木霉、长枝木霉、深绿木霉和粘绿木霉4个种。研究结果还表明,马铃薯根际土壤中的优势生防木霉菌种为哈茨木霉和长枝木霉,长枝木霉与深绿木霉对病原菌接骨木镰刀菌的拮抗作用最强。长枝木霉菌对接骨木镰刀菌有较好的拮抗效果,在国内系首次报道。
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| 关 键 词： | 马铃薯 根围土壤 木霉菌 干腐病 拮抗作用 |
| 收稿时间： | 1900-01-01; |
Screening of biocontrol Trichoderma from potato rhizosphere of Western Gansu against Fusarium sambucinum and their identification |
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| ZHANG Ru,LI Jin-hua,CHAI Zhao-xiang,WANG Di.Screening of biocontrol Trichoderma from potato rhizosphere of Western Gansu against Fusarium sambucinum and their identification[J].Acta Prataculturae Sinica,2009,18(2):138-145. |
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| Authors: | ZHANG Ru LI Jin-hua CHAI Zhao-xiang WANG Di |
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| Institution: | 1.Gansu Key Lab of Crop improvement and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, China; 2.College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 3.College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China |
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| Abstract: | The 108 isolates of Trichoderma were acquired from potato rhizosphere in farming-pasturing zone of Western Gansu,10 isolates of them had been acquired via the method of face to face on PDA to the pathogen Furuisam sambucinum causing potato dry rot.Through the identification based on morphology and analysis of ITS sequencing,these 10 isolates were identified as 4 species including Trichoderma harzianum,T.longibrachiatum,T.atroviride and T.virens.T.harzianum and T.longibrachiatum are super Trichoderma species... |
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| Keywords: | potato rhizosphere soil Trichoderma potato dry rot inhibition |
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