| 灰树花多糖提取工艺的研究 |
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| 引用本文： | 刘炳福,宫志远,张华,等.灰树花多糖提取工艺的研究[J].黑龙江农业科学,2014(3):104-106. |
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| 作者姓名： | 刘炳福 宫志远 张华 等 |
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| 作者单位： | [1]山东省农业科学院科技信息研究所,山东济南250100 [2]山东省农业科学院农业资源与环境研究所,山东济南250100 [3]山东中医药大学,山东济南250352 |
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| 基金项目： | 国家食用菌产业技术体系资助项目（CARS-24） |
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| 摘 要： | 为进一步优选灰树花多糖的最佳提取工艺,研究了溶剂用量、提取时间及提取次数对灰树花多糖提取的影响。结果表明:通过提取方法的考查确定葡糖糖吸收曲线为Y=0.125 8 X+0.105 5(R2=0.999 2),浓度在0.021 4~0.128 4mg·mL-1时与吸收度呈良好的线性关系。采用正交设计,用硫酸-蒽酮法在波长618nm处测定其吸收度,通过灰树花多糖得率的计算最终确定灰树花多糖的最佳提取工艺为水煎煮2次,煎煮时间分别为第1次提取2.0h,第2次提取1.0h,加28倍水,该方法简单,重现性好,提取率高。
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| 关 键 词： | 灰树花 多糖 提取工艺 硫酸—蒽酮法 |
Study on Extract Technique of Polysaccharide in Griflola frondosa |
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| LIU Bing-fu,GONG Zhi-yuan,ZHANG Hua,YAO Qiang.Study on Extract Technique of Polysaccharide in Griflola frondosa[J].Heilongjiang Agricultural Science,2014(3):104-106. |
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| Authors: | LIU Bing-fu GONG Zhi-yuan ZHANG Hua YAO Qiang |
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| Institution: | 1. Institute of Scientific Information, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100;2. Institute of Agricultural Resources and Environment, Shandong Acade- my of Agricultural Sciences,Jinan,Shandong 250100; 3. Shandong University of Traditional Chinese medlcine,Jinan, Shandong 250352) |
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| Abstract: | In order to optimize the best extract technique for Polysaccharide of Griflola frondosa ,the effect of dosage of solvent, extraction time and times on extracting of Griflola frondosa were studied. The results showed that through the determine of extraction method of polysaccharide, the amylose sorption degree lineari- tyrelation was Y=0. 1258X＋0. 105 5（R2 =0. 999 2）,the range of concentration was in 0. 021 4 0. 128 4 nag. mL-1 ,and had good linearity relation with absorbance. Orthogonal experiment was adopt, the absorbance was measured by anthrone-sulfuric acid at 618 nm. The best technique for Griflola frondosa lmlysaccharide was boil- ing 2 times,decoction time was 2.0 h first and then 1.0 h,amount of added water were 28 times. The simple method which was good for extracting of polysaccharide had good reproducibility and high extraction efficiency. |
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| Keywords: | Griflola frondosa polysaccharide extract technique anthrone-sulfuric acid |
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