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| 水稻类钙调磷酸酶B亚基蛋白基因OsCBL8功能的初步研究 | |
| 引用本文： | 马伯军,顾志敏,唐海娟,陈析丰,刘峰,张红生.水稻类钙调磷酸酶B亚基蛋白基因OsCBL8功能的初步研究[J].中国水稻科学,2009,23(2):127. |
| 作者姓名： | 马伯军 顾志敏 唐海娟 陈析丰 刘峰 张红生 |
| 作者单位： | 1. 南京农业大学,作物遗传与种质创新国家重点实验室,江苏,南京,210095;浙江师范大学,化学与生命科学学院,浙江,金华,321004 2. 南京农业大学,作物遗传与种质创新国家重点实验室,江苏,南京,210095 3. 浙江师范大学,化学与生命科学学院,浙江,金华,321004 |
| 基金项目： | 教育部长江学者和创新团队发展计划，教育部高等学校博士学科点专项科研基金，国家自然科学基金，浙江省自然科学基金 |
| 摘 要： | 以转正义或反义OsCBL8基因的T3代株系及非转基因对照为材料, 研究了转OsCBL8基因对水稻农艺性状、内源OsCBL8基因表达和幼苗耐盐、耐旱及耐冷性的影响。结果表明, 供试的16个转基因株系的株高、单株分蘖数、单株有效穗数、穗长和百粒重等性状与对照无显著差异, 每穗实粒数和结实率则大都显著或极显著低于对照。部分转基因株系的半定量RT PCR结果表明, 转正义基因显著增强了植株中OsCBL8基因的表达, 而转反义基因则对内源OsCBL8基因的表达具有一定的抑制作用。在盐、干旱和低温等非生物胁迫条件下的生长实验发现, 1个转正义基因株系的耐盐性得到显著增强, 1个转反义基因株系的耐旱性得到显著增强。 讨论了OsCBL8基因在水稻耐非生物逆境方面可能的机理。 |
| 关 键 词： | 水稻 转基因植株 耐逆性 分子机理 |
| 收稿时间： | 1900-01-01; |
Preliminary Study on the Function of Calcineurin B-Like Protein Gene OsCBL8 in Rice |
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| MA Bo-jun,GU Zhi-min,TANG Hai-juan,CHEN Xi-feng,LIU Feng,ZHANG Hong-sheng.Preliminary Study on the Function of Calcineurin B-Like Protein Gene OsCBL8 in Rice[J].Chinese Journal of Rice Science,2009,23(2):127. | |
| Authors: | MA Bo-jun GU Zhi-min TANG Hai-juan CHEN Xi-feng LIU Feng ZHANG Hong-sheng |
| Institution: | State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China; *Corresponding author, E-mail: hszhang@njau.edu.cn |
| Abstract: | The homozygous T3 transgenic lines with sense OsCBL8 and anti sense OsCBL8 obtained by Agro transformation were used to investigate the function of OsCBL8 in rice. The results showed that either over expression or inhibition of OsCBL8 gene in transgenic lines had little effect on main agronomic traits, but significantly decreased the number of filled grains per panicle and seed setting rate in some lines. Semi quantitative RT PCR showed that the expression of OsCBL8 extremely increased in sense transgenic seedlings, and decreased to some extent in anti sense transgenic ones. By evaluation of tolerance to 150 mmol/L NaCl, 20% PEG6000 and low temperature treatments, one sense transgenic line with high salt tolerance and one antisense transgenic line with high drought tolerance were obtained, respectively. It is discussed that the OsCBL8 gene may play an important role in rice tolerance to abiotic stresses. |
| Keywords: | rice transgenic plant tolerance molecular mechanism |
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