| 土壤颉颃芽孢杆菌B-Q2-7的分离筛选及其抑菌作用研究 |
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| 引用本文： | 全鑫,安德荣,孙德茂,马晓东,陈丽.土壤颉颃芽孢杆菌B-Q2-7的分离筛选及其抑菌作用研究[J].西北农业学报,2007,16(3):252-256. |
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| 作者姓名： | 全鑫 安德荣 孙德茂 马晓东 陈丽 |
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| 作者单位： | 西北农林科技大学植保学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100 |
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| 基金项目： | 教育部长江学者和创新团队发展计划 |
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| 摘 要： | 从杨凌大寨乡采来的土壤中分离到142株芽孢杆菌,经过皿内对峙初筛和无菌发酵滤液复筛得到一株对稻瘟病菌,小麦赤霉病菌,黄瓜枯萎病菌,梨黑星病菌,烟草赤星病菌,番茄灰霉病菌均具有强颉颃作用的芽孢杆菌B-Q2.单孢颉颃性筛选表明B-Q2-7的抑菌活性最强,对稻瘟病菌和番茄灰霉病菌的抑制率分别达到87.4%和90.6%.盆栽实验结果显示B-Q2-7无菌发酵滤液对番茄灰霉病具有良好的预防和治疗作用,10倍液的预防效果和治疗效果分别为89.6%和80.4%.抑菌机理研究表明,B-Q2-7代谢产物对梨黑星和番茄灰霉病菌的孢子萌发具有极强的抑制作用,在稀释10倍时的抑制萌发率分别达到95.9%和100%;显微观察到被B-Q2-7抑制的病原真菌菌丝大部分扭曲、肿大、畸形,出现膨大的泡囊.
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| 关 键 词： | 芽孢杆菌 分离筛选 抑制率 防治效果 菌丝 |
| 文章编号： | 1004-1389（2007）03-0252-05 |
| 收稿时间： | 2006-09-24 |
| 修稿时间： | 2006-09-242006-12-09 |
Study on Isolation, Screening of Antagonistic Bacillus B-Q2-7 from Soil and Its Inhibitory Efficacy |
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| QUAN Xin,AN De-rong,SUN De-mao,MA Xiao-dong and CHEN Li.Study on Isolation, Screening of Antagonistic Bacillus B-Q2-7 from Soil and Its Inhibitory Efficacy[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(3):252-256. |
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| Authors: | QUAN Xin AN De-rong SUN De-mao MA Xiao-dong and CHEN Li |
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| Institution: | Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China;Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China;Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China;Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China;Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | There were 142 bacillus strains isolated from soil,which collected in the village of Dazhai,Yangling,Shaanxi province.By plate test and cell-free fermentation filtrate screening,B-Q2 was obtained which is a stronger antagonistic bacillus and can inhibit Magnaporthe grisea,Fusarium graminearum,Fusarium oxysporum,Venturia nashicola,Alternaria alternate and Botrytisci nerea.The screening of single-spore inhibiting activity showed that the inhibiting activity of B-Q2-7 was the best,the inhibition rate on Magnaporthe grisea and Botrytisci nerea could reach 87.4% and 91.6% respectively.Results of pot experiment showed that cell-free fermentation filtrate of B-Q2-7 had strong preventing and cure efficacy on Botrytisci nerea,the preventing and cure effect could get to 89.6% and 80.4% respectively when fermentation filtrate was diluted 100 times.Study on inhibiting mechanism of strain B-Q2-7 demonst rated that metabolites produced by antagonistic strain B-Q2-7 could strongly inhibit the sporus germination of Venturia nashicola and Botrytisci nerea.It''s inhibition rate of germination could reach 95.9% and 100% when fermentation filtrate was diluted 100 times.Oberserving by microscope showed that the mycelium inhibited by strainB-Q2-7 mostly twisted,inflated,deformed and formed expanded vacuole. |
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| Keywords: | Bacillus Isolate-screening Inhibition rate Control-effect Mycelium |
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