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| Sry基因和Y染色体重复序列在牛早期胚胎性别鉴定中应用效果的比较 | |
| 引用本文： | 张伟,王世银,张兆旺,赵兴绪.Sry基因和Y染色体重复序列在牛早期胚胎性别鉴定中应用效果的比较[J].中国奶牛,2011(16):5-8. |
| 作者姓名： | 张伟 王世银 张兆旺 赵兴绪 |
| 作者单位： | 1. 新疆农业职业技术学院动物科技学院,昌吉,831100 2. 新疆农业职业技术学院继续教育学院,昌吉,831100 3. 甘肃农业大学动物科学技术学院,兰州,730070 4. 甘肃农业大学动物医学院,兰州,730070 |
| 基金项目： | 甘肃省科技厅资助项目《家畜胚胎规模化生产及相关新技术的产业化开发》 |
| 摘 要： | 本试验以牛Sty基因和Y染色体重复序列作为雄性特异性基因，分别设计引物，建立多重巢式PCR体系，比较二者在牛早期胚胎性别鉴定中的应用效果。试验结果表明，当扩增体系中的模板量为一个胚胎细胞的DNA量时，以Y染色体重复序列构建的扩增体系比Sry基因具有更高的灵敏度和稳定性，更适合用于牛早期胚胎性别鉴定。 |
| 关 键 词： | Sry基因 Y染色体重复序列 胚胎性别鉴定 牛 PCR法 |
Comparison of the Effects of Nest PCR System Established by Sry Gene and Y Chromosome Repeated Sequence for Sex Determination of Bovine Embryo |
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| ZHANG Wei,WANG Shi-yin,ZHANG Zhao-wang,ZHAO Xing-xu.Comparison of the Effects of Nest PCR System Established by Sry Gene and Y Chromosome Repeated Sequence for Sex Determination of Bovine Embryo[J].China Dairy Cattle,2011(16):5-8. | |
| Authors: | ZHANG Wei WANG Shi-yin ZHANG Zhao-wang ZHAO Xing-xu |
| Institution: | 1. Faculty of Animal Science and Technology, XATC, Changji 831100;2. Faculty of Further Education, XATC, Changji 831100; 3. Faculty of Animal Science and Technology, GAU, Lanzhou 730070;4. Faculty of Veterinary Medicine, GAU, Lanzhou 730070) |
| Abstract: | Sty gene and Y chromosome repeated sequence were selected as male-specific gene for sex determination of bovine embryo in this study. Eight pairs of primers were designed and a nest PCR system was established in order to compare the amplification effect. The results showed that the system established by Y chromosome repeated sequence was more sensitive and stable than the one established by Sty gene when the PCR template was the DNA from one embryonic ceil, so it was more suitable for sex determination of bovine embryo. |
| Keywords: | Sry gene Y chromosome repeated sequence Sex determination Bovine embryo PCR |
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