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海洋低温碱性蛋白酶的皮肤变态反应研究
引用本文:韩彦弢,仲伟珍,王玉贞,闫凤霞,于业军,刘晓萍,张云波,王跃军,王春波.海洋低温碱性蛋白酶的皮肤变态反应研究[J].海洋水产研究,2000,21(4):102-105.
作者姓名:韩彦弢  仲伟珍  王玉贞  闫凤霞  于业军  刘晓萍  张云波  王跃军  王春波
作者单位:1. 青岛大学医学院,266021
2. 青岛大学医学院附属医院,266003
3. 中国水产科学研究院黄海水产研究所 海洋酶与酶工程开放实验室,青岛 266071
基金项目:国家“海洋863”项目(863-819-06-01)资助
摘    要:利用酶工程技术制得的海洋低温碱性蛋白酶,主要用于加酶的洗涤剂,在室温下有较高的酶活力。为确保海洋低温碱性蛋白酶使用的安全性,并为其应用提供实验依据,本文以豚鼠为受试动物,进行皮肤致敏反应试验。结果表明,经致接触和激发接触,2,4-二硝基氯苯组(DNCB)皮肤最大平均反应值为3.3,致敏率为100%。海洋低温碱性蛋白酶组皮肤最大平均反应值为0.05,致敏率为5%。提示海洋低温碱性蛋白酶为弱致敏物。

关 键 词:海洋低温碱性蛋白酶  豚鼠  皮肤变态反应
修稿时间:2000年11月4日

Study on the skin allergy reaction of the marine low-temperature alkaline proteinase
HAN Yan tao,ZHONG Wei zhen,WANG Yu zhen,YAN Feng xia,YU Ye jun,LIU Xiao pin,ZHANG Yun bo,WANG Yue jun,WANG Chun bo.Study on the skin allergy reaction of the marine low-temperature alkaline proteinase[J].Marine Fisheries Research,2000,21(4):102-105.
Authors:HAN Yan tao  ZHONG Wei zhen  WANG Yu zhen  YAN Feng xia  YU Ye jun  LIU Xiao pin  ZHANG Yun bo  WANG Yue jun  WANG Chun bo
Institution:HAN Yan tao 1 ZHONG Wei zhen 1 WANG Yu zhen 1 YAN Feng xia 1 YU Ye jun 2 LIU Xiao pin 1 ZHANG Yun bo 3 WANG Yue jun 3 WANG Chun bo 1
Abstract:The Marine low temperature alkaline proteinase is made by enzyme engineering technology. It is mainly used as detergent and has very high activity at room temperature. In order to ensure the safety of the Marine low temperature alkaline proteinase usage and provide experimental data for it, it is used to do the skin sensitization test on guinea pigs. The results show the skin maximum average value of the reaction in 2,4 diniiro chlorobeuzene(DNCB)group is 3 3 and the allergic reaction rate is 100% by sensitization and excited contact. The skin maximum average value of the reaction in Marine low temperature alkaline proteinase group is 0 05 and the allergic reaction rate is 5%. It is a sensitized.
Keywords:Marine  low  temperature alkaline Proteinase    Guinea pigs    Skin sensitization  
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