| 龙牙楤木组织培养与快速繁殖技术研究 |
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| 引用本文： | 秦亚平,吴大椿,肖锦和.龙牙楤木组织培养与快速繁殖技术研究[J].安徽农业科学,2004,32(1):111-114. |
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| 作者姓名： | 秦亚平 吴大椿 肖锦和 |
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| 作者单位： | 湖北农学院,湖北荆州,434025;湖北农学院,湖北荆州,434025;湖北农学院,湖北荆州,434025 |
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| 摘 要： | 对龙牙木匆心 木 Araliaelata (Miq)Seen]的叶片、叶柄和幼茎进行组织培养 ,结果表明 :用叶片进行组织培养的效果最好 ,愈伤组织最高诱导率达 3 7.1%;最适诱导培养基为MS +6-BA 1.0mg/L +2 ,4-D 1.0mg/L ;最适分化培养基为MS +6-BA 2 .0mg/L +2 ,4-D 1.0mg/L ;最好的继代增殖材料为幼根。试验还发现 :用 40 μg/L的硫酸链霉素可以有效防止细菌的污染 ;5g/L的活性炭防止褐变效果最好 ;把握好琼脂的用量及适当降低 6-BA的含量可以降低玻璃化现象的发生频率。
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| 关 键 词： | 龙牙木楤木 组织培养 愈伤组织 |
| 文章编号： | 0517-6611(2004)01-0111-04 |
Study on Tissue Culture and Quickly Reproduction Technology of Aralia mandshurica |
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| Qing Yaping et al.Study on Tissue Culture and Quickly Reproduction Technology of Aralia mandshurica[J].Journal of Anhui Agricultural Sciences,2004,32(1):111-114. |
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| Authors: | Qing Yaping |
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| Abstract: | Leaf, leafstalk and young caudex culture of Aralia elata (Miq) Seen were experimented.The result showed that, the leaf tissue could get the best result, inducement rate of reproductive tissue could reach to 37.1%. The best culture medium was MS+6BA 1.0 ?mg/L+2,4-D 1.0 ?g/L; the best polarization culture medium was MS+6-BA 2.0 mg/L+2,4-D 1.0 mg/L.The experiment indicated that streptomycin sulfate of 40?μg/ml could prevent germ contamination. Active carbon of 5?g/L could prevent culturing materials from browned and vetrification could become less frequent if the content of 6-BA was reduced. |
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| Keywords: | Aralia elata(Miq) Seen Tissue culture Reproductive tissue |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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