| 丹参花药愈伤组织诱导因素的研究 |
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| 引用本文： | 解玉丽,王建华,宋振巧,马晓冲.丹参花药愈伤组织诱导因素的研究[J].山东农业科学,2010(8):5-7,10. |
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| 作者姓名： | 解玉丽 王建华 宋振巧 马晓冲 |
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| 作者单位： | 山东农业大学药用植物研究室,山东,泰安,271018 |
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| 基金项目： | 山东省农业良种工程产业化项目 |
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| 摘 要： | 研究了不同激素处理、不同花粉发育时期、不同低温预处理和高温预培养对丹参花药愈伤组织诱导的影响。结果表明：最佳的培养基激素组合为MS＋2.0 mg/L6-BA＋0.2 mg/L NAA;单核靠边期的花药愈伤组织诱导率最高;4℃低温预处理2 d最有助于愈伤组织发生;高温预培养不利于愈伤组织诱导率的提高。
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| 关 键 词： | 丹参 花药培养 愈伤组织 |
Study of Influential Factors on Anther Callus Induction of Salvia miltiorrhiza |
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| XIE Yu-li,WANG Jian-hua,SONG Zhen-qiao,MA Xiao-chong.Study of Influential Factors on Anther Callus Induction of Salvia miltiorrhiza[J].Shandong Agricultural Sciences,2010(8):5-7,10. |
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| Authors: | XIE Yu-li WANG Jian-hua SONG Zhen-qiao MA Xiao-chong |
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| Institution: | XIE Yu-li,WANG Jian-hua,SONG Zhen-qiao,MA Xiao-chong(Laboratory of Medicinal Plant,Shandong Agricultural University,Taian 271018,China) |
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| Abstract: | Several factors such as different hormone treatments,pollen developmental stages,low-temperature pretreatment and high-temperature pre-incubation were studied on anther callus induction of Salvia miltiorrhiza.The results showed that 2.0 mg/L 6-BA + 0.2 mg/L NAA was the best hormone combination;the highest anther callus inductivity appeared at the uninucleate eccentric stage;the pretreatment at 4℃ for 2 days was the most conductive to callus occurrence,while pre-incubation at 35℃ was not conductive to the increase of callus induction rate. |
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| Keywords: | Salvia miltiorrhiza Anther culture Callus |
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