| 高羊茅辐射敏感性和辐照处理对其成熟种子愈伤诱导的影响 |
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| 引用本文： | 吴关庭,胡张华,陈笑芸,郎春秀,陈锦清,夏英武.高羊茅辐射敏感性和辐照处理对其成熟种子愈伤诱导的影响[J].核农学报,2004,18(2):104-106,112. |
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| 作者姓名： | 吴关庭 胡张华 陈笑芸 郎春秀 陈锦清 夏英武 |
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| 作者单位： | 浙江大学原子核农业科学研究所,浙江,杭州,310029;浙江省农业科学院病毒学与生物技术研究所,浙江,杭州,310021;浙江省农业科学院病毒学与生物技术研究所,浙江,杭州,310021;浙江大学原子核农业科学研究所,浙江,杭州,310029 |
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| 摘 要： | 无菌培养条件下发芽率、成苗率、苗高和根长的测定结果表明 ,4个高羊茅品种的辐射敏感性差异很大 ,美洲虎 3号和可奇思对辐射比较敏感 ,半致死剂量大约在 1 5 0Gy左右 ,凌志和猎狗 5号的辐射敏感性分别中等和较弱。用低剂量γ射线处理可促进高羊茅成熟种子的愈伤形成 ,而且诱导愈伤中胚性愈伤的比例有一定提高。因此 ,成熟种子辐照处理可作为提高高羊茅组培效率的一种辅助手段。
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| 关 键 词： | 高羊茅 辐射敏感性 辐照处理 愈伤诱导 胚性愈伤 |
| 文章编号： | 1000-8551(2004)02-104-03 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
STUDY ON RADIATION SENSITIVITY AND INFLUENCE OF GAMMA- RAY TREATMENT ON CALLUS INDUCTION FROM MATURE SEEDS OF TALL FESCUE |
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| WU Guan-ting , HU Zhang-hua CHEN Xiao-yun LANG Chun-xiu CHEN Jin-qing XIA Ying-wu.STUDY ON RADIATION SENSITIVITY AND INFLUENCE OF GAMMA- RAY TREATMENT ON CALLUS INDUCTION FROM MATURE SEEDS OF TALL FESCUE[J].Acta Agriculturae Nucleatae Sinica,2004,18(2):104-106,112. |
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| Authors: | WU Guan-ting HU Zhang-hua CHEN Xiao-yun LANG Chun-xiu CHEN Jin-qing XIA Ying-wu |
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| Institution: | WU Guan-ting 1,2 HU Zhang-hua 2 CHEN Xiao-yun 2 LANG Chun-xiu 2 CHEN Jin-qing 2 XIA Ying-wu 1 |
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| Abstract: | Measurement of seed germination, seedling survival and height, and root length under the condition of sterile culture indicated that four tall fescue cultivars varied greatly in radiation sensitivity. Jaguar 3 and Cochise were sensitive to radiation with a median lethal dose (LD 50 ) of about 150Gy, whereas Barlexas and Houndog 5 had moderate and weak radiation sensitivity respectively. Callus induction from mature seeds of tall fescue was stimulated and the ratio of embryogenic calli to induced calli increased to a certain extent by treatment with low dose γ-rays. It was proposed, therefore, that irradiation treatment of mature seeds could be used as a supplementary means of enhancing tissue culture efficiency of tall fescue. |
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| Keywords: | tall fescue(Festuca arundinacea Schreb ) radiation sensitivity irradiation treatment callus induction embryogenic callus |
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