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唐菖蒲球茎芽高频再生体系的建立
引用本文:艾丽皎,李名扬.唐菖蒲球茎芽高频再生体系的建立[J].西南农业大学学报,2005,27(6):844-846.
作者姓名:艾丽皎  李名扬
作者单位:[1]重庆市园林绿化科学研究所,重庆400038 [2]西南大学花卉研究所,重庆400716
摘    要:将带芽的唐菖蒲子球茎切块接种在附加2,4-D3.0mg/L的MS基本培养基上诱导愈伤组织的发生。愈伤组织转至MS+6-BA0.5mg/L+NAA0.1mg/L诱导芽的分化。丛生芽继代增殖在MS+6-BA0.5mg/L+NAA0.1mg/L上,增殖系数最高。生根培养以1/2MS+NAA0.1mg/L效果最佳。

关 键 词:唐菖蒲  球茎  芽增殖  再生  组织培养
文章编号:1000-2642(2005)03-0844-03
收稿时间:2005-01-13
修稿时间:2005-01-13

ESTABLISHMENT OF A HIGH FREQUENCY REGENERATION SYSTEM OF GLADIOLUS CORM BUDS
AI Li - jiao, LIMing - yang.ESTABLISHMENT OF A HIGH FREQUENCY REGENERATION SYSTEM OF GLADIOLUS CORM BUDS[J].Journal of Southwest Agricultural University,2005,27(6):844-846.
Authors:AI Li - jiao  LIMing - yang
Institution:1. Gardens Virescence Science Reseorch Institute, Chongqing 400038, China; 2. Flower Research Institute, Southwest University, Chongqing 400716, China
Abstract:Slices of dormant cormlets with buds of Gladiolus hybridus were used as explants and inoculated on MS basal medium supplemented with 2,4-D 3.0 mg/L for callus induction.The resulting calli were transferred to media of shoot differentiation and proliferation containing different combinations of 6-BA and NAA.The optimum media for shoot differentiation,proliferation and rooting were MS + 6BA 0.5 mg/L + NAA 0.1 mg/L,MS + 6-BA 0.5 mg/L + NAA 0.1 mg/L and 1/2 MS + NAA 0.1 mg/L.
Keywords:Gladiolus hybridus  corm  bud multiplication  regeneration  tissue culture
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