| 利用花粉管通道法将兔防御素NP-1基因导入小麦的研究 |
| |
| 引用本文： | 周春江,葛荣朝,赵宝存,黄占景,沈银柱,何聪芬.利用花粉管通道法将兔防御素NP-1基因导入小麦的研究[J].华北农学报,2007,22(2):26-28. |
| |
| 作者姓名： | 周春江 葛荣朝 赵宝存 黄占景 沈银柱 何聪芬 |
| |
| 作者单位： | 1. 河北师范大学生命科学学院,河北,石家庄,050016
2. 北京工商大学,化工学院,北京市植物资源研究开发重点实验室,北京,100037 |
| |
| 摘 要： | 兔防御素NP-1是抗性谱最广的防御素之一。为提高小麦的抗病虫能力,培育优质高抗的小麦品种,采用花粉管通道法将兔防御素NP-1基因导入优质小麦品种G8901。对得到的193株T0植株进行抗除草剂筛选,获得4株抗性植株。通过PCR及PCR-Southern杂交,证实了NP-1基因已经整合到转基因植株的基因组中,并稳定遗传到T1。田间抗病虫鉴定结果表明,这些植株对于白粉病、叶锈和条锈病有较强的免疫力和抗性,但对于蚜虫的抗性没有明显提高。
|
| 关 键 词： | 小麦 兔防御素NP-1基因 转化 花粉管通道 |
| 文章编号： | 1000-7091（2007）02-0026-03 |
| 修稿时间： | 2006-09-28 |
Study on Transgenic Wheat with Rabbit Defensin(NP-1)via the Pollen-tube Pathway |
| |
| ZHOU Chun-jiang,GE Rong-chao,ZHAO Bao-cun,HUANG Zhan-jing,SHEN Yin-zhu,HE Cong-fen.Study on Transgenic Wheat with Rabbit Defensin(NP-1)via the Pollen-tube Pathway[J].Acta Agriculturae Boreali-Sinica,2007,22(2):26-28. |
| |
| Authors: | ZHOU Chun-jiang GE Rong-chao ZHAO Bao-cun HUANG Zhan-jing SHEN Yin-zhu HE Cong-fen |
| |
| Institution: | 1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China; 2. College of Chemistry and Environment Engineering, Beijing Key Lab of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100037, China |
| |
| Abstract: | Rabbit defensin NP-1gene has a broad resistance spectrum to pathogens.In order to obtain high disease and insect resistance transgenic wheat,rabbit defensin NP-1gene was introduced into the wheat cultivar of G8901,via the pollen-tube pathway.Four transgenic plants were obtained by screening of 193 T0 generation plants with herbicide.Genomic PCR and PCR-Southern revealed that NP-1 gene was integrated into the genome of these transgenic plants,and it was transmitted to T1 generation stably.The results of disease resistance and insect resistance appraisal showed that these plants had got higher resistance to powdery mildew,leaf rust and stripe rust,but the resistance to aphid had not been improved,which revealed that NP-1 gene had expressed in wheat genome. |
| |
| Keywords: | Wheat Rabbit defensin NP-1 gene Transgenic Pollen-tube pathway |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
