| 基于线粒体DNA序列探讨中国紫貂的起源 |
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| 引用本文： | 徐纯柱,张洪海,马建章,刘忠华.基于线粒体DNA序列探讨中国紫貂的起源[J].黑龙江畜牧兽医,2011(15). |
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| 作者姓名： | 徐纯柱 张洪海 马建章 刘忠华 |
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| 作者单位： | 东北农业大学生命科学学院;曲阜师范大学生命科学学院;东北林业大学野生动物资源学院; |
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| 基金项目： | 国家教育部新世纪优秀人才支持计划资助项目(NCET-070507); 东北农业大学引进人才项目(2010RCB50); 黑龙江省科技攻关项目(PC07S03) |
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| 摘 要： | 为了研究中国紫貂的起源,试验利用PCR方法和直接测序技术对中国境内分布的18只野生紫貂线粒体DNA控制区全序列进行了测定和群体分析。结果表明:所获得的7个单倍型序列长度介于1 073～1 134 bp之间,在保守序列区CSB1与CSB2之间存在AC串连重复序列,长度介于198～258 bp,这是导致紫貂线粒体控制区序列长度异质性的原因;序列中具119个碱基插入/缺失位点、2个颠换位点以及26个转换位点;其整体单倍型多样性(H)为0.880±0.045、核苷酸多样性(Pi)为0.039±0.004,种群总体遗传多样性水平较高。3个地理亚种间存在明显的遗传分化,尤其是长白山和阿尔泰亚种间FST(遗传分化指数)=0.989 49(P>0.01),种群间基因流极低(Nm=0.005);阿尔泰亚种、长白山亚种分别与大兴安岭亚种的个别种群存在较近的亲缘关系,该结论与紫貂化石证据结合即可推测出紫貂大兴安岭亚种是我国境内分布的3个紫貂种群中首先分化形成的,然后以大兴安岭为中心向南分化形成长白山亚种以及向西分化形成阿尔泰亚种。
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| 关 键 词： | 紫貂 线粒体DNA 控制区 遗传多样性 |
lnvestigating the origin of M.zibellina in China based on mitochondrial sequence data |
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| XU Chun-zhu,ZHANG Hong-hai,MA Jian-zhang,LIU Zhong-hua.lnvestigating the origin of M.zibellina in China based on mitochondrial sequence data[J].Heilongjiang Animal Science And veterinary Medicine,2011(15). |
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| Authors: | XU Chun-zhu ZHANG Hong-hai MA Jian-zhang LIU Zhong-hua |
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| Institution: | XU Chun-zhu1,ZHANG Hong-hai2,MA Jian-zhang3,LIU Zhong-hua1(1.College of Life Sciences,Northeast Agricultural University,Harbin 150030,China,2.College of Life Sciences,Qufu Normal University,Qufu 273165,3.College of Wild life Resource,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | To study the origin of M.zibella in China,The mitochondrial DNA control regions of 18 wild Sables collected in China were sequenced by polymerase chain reaction(PCR) and analysed.The results showed that the complete control regions in mt DNA from 7 haplotypes were located at 1073-1134 bp.There were AC repeat sequences located at 198-258 bp fragment in conserved sequence block domain,which caused the sequence length heterogeneity of mitochondrial DNA control regions of Sable.There were 119 base insertion/del... |
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| Keywords: | M zibellina mt DNA control region genetic diversity |
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