| 猪肉及其制品中几种病原微生物芯片检测方法的建立及应用 |
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| 引用本文： | 肖进文,李应国,刘生峰,王昱,聂福平,谭志,臧庆伟,邓朝晖.猪肉及其制品中几种病原微生物芯片检测方法的建立及应用[J].安徽农业科学,2010,38(18):9478-9480,9607. |
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| 作者姓名： | 肖进文 李应国 刘生峰 王昱 聂福平 谭志 臧庆伟 邓朝晖 |
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| 作者单位： | 重庆出入境检验检疫局,重庆,400020;博奥生物有限公司,北京,102206 |
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| 摘 要： | 目的]建立金黄色葡萄球菌、沙门氏菌、大肠杆菌O157：H7、单增李斯特氏菌和空肠弯曲杆菌检测的基因芯片方法,并在实际应用中检测芯片的效果。方法]以5种目标菌保守基因片段为模板设计引物,用待检样品增菌后提取的DNA模板进行2个独立的多重PCR扩增。扩增产物与固定有5种目标致病菌特异性探针的基因芯片进行杂交,用芯片扫描仪对芯片杂交结果进行扫描并判定结果。结果]使用基因芯片检测5种细菌的检测下限分别为：金黄色葡萄球菌8.7×105cell/ml;大肠杆菌O157：H7 5.0×105cell/ml;沙门氏菌4.4×105cell/ml;单增李斯特氏菌2.4×105cell/ml;空肠弯曲杆菌6.2×106cell/ml。结论]与传统方法比较,芯片法具有较高的特异性和灵敏性,是一种能应用于实际的快速高通量检测细菌的方法。
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| 关 键 词： | 病原微生物 芯片法 传统检测法 猪肉 |
Establishment and Application of Chip Detection Method of Several Pathogenic Microorganisms in Pork and Their Products |
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| XIAO Jin-wen et al.Establishment and Application of Chip Detection Method of Several Pathogenic Microorganisms in Pork and Their Products[J].Journal of Anhui Agricultural Sciences,2010,38(18):9478-9480,9607. |
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| Authors: | XIAO Jin-wen |
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| Institution: | XIAO Jin-wen et al(Chongqing Entry-exit Inspection and Quarantine Bureau,Chongqing 400020) |
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| Abstract: | Objective] To establish gene chip detection method for testing Staphylococcus aureus,Salmonella,E.coli O157: H7,Tenzin Listeria monocytogenes and Campylobacter jejuni,and to test the effect of the chip in practical application.Method] The conservative gene fragment of 5 kinds of bacteria was used as templates to design primers,the number of bacteria samples to be detected was increased,and DNA extracted from the samples as template for two separate multiple PCR amplification.Then PCR products was hybridiz... |
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| Keywords: | Pathogenic microorganisms Chip detection method Traditional detection method Pork |
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