| HPLC法测定醋酸氯己定子宫灌注液的含量 |
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| 引用本文： | 周艳飞.HPLC法测定醋酸氯己定子宫灌注液的含量[J].中国兽药杂志,2010,44(5):31-32. |
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| 作者姓名： | 周艳飞 |
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| 作者单位： | 北京市兽药监察所,北京,100107 |
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| 摘 要： | 建立了测定醋酸氯己定含量的高效液相色谱法。以C18(4.6 mm×150 mm,5μm)为色谱柱,0.02 mol/L磷酸二氢钾溶液(pH2.6)-乙腈(70∶30)为流动相,流速1 mL/min,检测波长254 nm。结果显示,醋酸氯己定在0.5～198μg/mL浓度范围内,浓度对峰面积的线性关系良好(r=0.999 98),平均回收率为96.40%(n=9)。实验表明,该方法灵敏、准确,可以较好地控制醋酸氯己定子宫灌注剂的质量。
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| 关 键 词： | HPLC法 醋酸氯己定 含量测定 |
| 收稿时间： | 2009/12/29 0:00:00 |
| 修稿时间： | 4/5/2010 12:00:00 AM |
Determination of Chlorhexidine Acetate in Uterus Perfusion Agent by HPLC |
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| zhouyanfei.Determination of Chlorhexidine Acetate in Uterus Perfusion Agent by HPLC[J].Chinese Journal of Veterinary Drug,2010,44(5):31-32. |
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| Authors: | zhouyanfei |
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| Institution: | ZHOU Yan-fei (Institute of Veterinary Dury Control,Beijing 100107,China) |
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| Abstract: | An HPLC method was established for the determination of chlorhexidine acetate in uterus perfusion agent.The analysis was carried on a column of C18（4.6 mm×150 mm,5 μm）,using 0.02 mol/L KH2PO4（pH2.6）-acetonitrile（70∶30）as the mobile phase with a flow rate of 1 mL/min,and UV detection at 254 nm.Results showed that a good linearity can be found in the range of 0.5～198 μg/mL（r=0.999 98）.The average recovery was 96.40%（n=9）.This method was more accurate and sensitive,and can be used to control the chlorhexidine acetate in uterus perfusion agent. |
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| Keywords: | HPLC chlorhexidine acetate content determination |
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