| 毛竹根部解磷细菌的筛选及多样性研究 |
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| 引用本文： | 韩烁,夏冬亮,李潞滨,韩继刚.毛竹根部解磷细菌的筛选及多样性研究[J].河北农业大学学报,2010,33(2). |
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| 作者姓名： | 韩烁 夏冬亮 李潞滨 韩继刚 |
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| 作者单位： | 1. 河北大学,生命科学学院,河北,保定,071002
2. 中国林业科学研究院,林业研究所,国家林业局林木培育重点实验室,北京,100091 |
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| 基金项目： | 上海市科委重大攻关计划(09dz1205001) |
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| 摘 要： | 本研究对毛竹(Phyllostachys pubescens)根部(包括根际、根面及内生细菌)解磷细菌进行了筛选,并通过16S rDNA序列分析对其多样性进行了初步研究。从179株毛竹根部细菌中筛选出26株解磷细菌,包括14株根际细菌、8株根面细菌、4株内生细菌。其中,芽孢杆菌BK24的解磷活性最高,其解磷活性达到369.06 mg/L。16S rDNA序列分析表明:26株解磷细菌中,13株属于厚壁菌门(Firmicutes,50.00%),5株属于变形菌门β亚群(Betaproteobacteria,19.23%),4株属于变形菌门γ亚群(Gammaproteobacteria,15.38%),4株属于放线菌门(Actinobacteria,15.38%)。芽孢杆菌属(Bacillus,38.46%)和伯克霍尔德氏菌属(Burkholderia,19.23%)为优势菌属。
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| 关 键 词： | 毛竹 解磷细菌 多样性 |
Diversity of the phosphate solubilizing bacteria isolated from the root of Phyllostachys pubescens |
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| HAN Shuo,XIA Dong-liang,LI Lu-bin,HAN Ji-gang.Diversity of the phosphate solubilizing bacteria isolated from the root of Phyllostachys pubescens[J].Journal of Agricultural University of Hebei,2010,33(2). |
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| Authors: | HAN Shuo XIA Dong-liang LI Lu-bin HAN Ji-gang |
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| Institution: | 1.College of Life Sciences;Hebei University;Baoding 071002;China;2.Research Institute of Forestry;Chinese Academy of Forestry;Key Laboratory of Tree Breeding and Cultivation;State Forestry Administration;Beijing 100091;China |
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| Abstract: | 26 phosphate-solubilizing bacteria were screened from 179 rhizosphere,rhizoplane and root endophytic bacterial strains of moso bamboo(Phyllostachys pubescens) plants.The phosphate-solubilizing bacteria included fourteen rhizobacteria isolates,eight rhizoplane bacteria isolates,and four endophytic bacteria isolates.Bacillus sp.BK24 showed the highest phosphate solubilizing activity(369.06 mg/L).Phylogenetic analysis based on the partial 16S rDNA sequences indicated that the strains were clustered into four g... |
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| Keywords: | Phyllostachys pubescens phosphate-solubilizing bacteria diversity |
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