| 家鸭GHSR基因部分编码序列的克隆及变异检测 |
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| 引用本文： | 詹凯,许月英,赵瑞宏.家鸭GHSR基因部分编码序列的克隆及变异检测[J].中国畜牧兽医,2008,35(6). |
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| 作者姓名： | 詹凯 许月英 赵瑞宏 |
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| 作者单位： | 安徽省农科院畜牧兽医研究所,合肥,230031 |
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| 基金项目： | 安徽省科技攻关项目
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| 摘 要： | 作者旨在克隆鸭GHSR基因mRNA部分编码区序列,并筛查克隆序列中的变异位点。采用RT-PCR法从巢湖鸭下丘脑组织中分离家鸭GHSR基因mRNA中编码区核酸序列,并选用30个个体cDNA,通过构建cDNA池对克隆编码区的序列变异测序检测。结果表明,克隆鸭GHSR基因mRNA部分编码区核酸序列长635 bp(GenBank登录号:EU005225),编码211个氨基酸,与鸡GHSR基因同源核酸相似性达到94%,氨基酸相似性为97%;cDNA池测序检测揭示克隆区段存在3个碱基变异位点,均为同义突变,未使编码氨基酸发生改变。克隆鸭GHSR基因mRNA编码区核酸、氨基酸序列与鸡同源序列的相似性,以及克隆核酸序列的变异检测结果表明,鸭GHSR基因在序列和功能上具有很高的保守性。
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| 关 键 词： | 鸭 mRNA克隆 cDNA池 变异 |
Molecular Cloning and Variation Detection of the Partial Coding Region of the Duck GHSR Gene |
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| ZHAN Kai,XU Yue-ying,ZHAO Rui-hong.Molecular Cloning and Variation Detection of the Partial Coding Region of the Duck GHSR Gene[J].China Animal Husbandry & Veterinary Medicine,2008,35(6). |
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| Authors: | ZHAN Kai XU Yue-ying ZHAO Rui-hong |
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| Abstract: | To get the partial coding region of the duck GHSR mRNA and to detect the mutation loci in the cloned sequence,RT-PCR was carried out to isolate the target sequence from the hypothalamic tissue of the native breed,Chaohu duck,and then cDNA pooling with 30 cDNA samples was amplified and sequenced for the mutation detection of the cloned sequence.The result showed the cloned mRNA sequence consists of 635 base pairs in length(GenBank Accession No.EU005225) and codes 211 amino acids,and the similarities of nucleic acid and amino acid of the GHSR gene between the duck and the chicken are 94% and 97%,respectively.Three synonymous mutations which don't change the corresponding amino acids were found by cDNA pooling in cloned coding region.The sequence and variation analysis in this experiment demonstrate that the high level of conservation exists in sequence and function of the duck GHSR gene. |
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| Keywords: | GHSR |
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