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Genetic Differences of Mitten Crabs Based on RFLP Analysis on Mitochondrial Cytochrome Oxidase Subunit I (CO I) Gene
作者姓名:HU Pengfei  ;WANG Qian  ;DAI Wei  ;WANG Xiaomei
作者单位:HU Pengfei1,2,WANG Qian1,DAI Wei1,and WANG Xiaomei1* 1 Tianjin Key Laboratory of Aqua-Ecology and Aquaculture,Fisheries Science Department,Tianjin Agricultural University,Tianjin 300384,China 2 College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China
基金项目:天津市自然科学基金,国际合作项目,天津高等教育基金
摘    要:The genetic differences of 15 mitten crab populations from 6 river systems in mainland China and 1 population from Russia were studied based on RFLP analysis of mitochondrial cytochrome oxidase subunit I (CO I). The results showed that Tas I-RFLP pattern could be used as a genetic marker to distinguish Eriocheir hepuensis from Eriocheir sinensis, Eriocheirjaponica and Eriocheir leptognathus; genetic distances among 13 populations of Eriocheir sinensis range from 0 to 0.015, indicating that they were different geographic strains; the subspecies status of Eriocheir sinensis and Eriocheir hepuensis (population from Nanliujiang) were considered owning to their genetic distances of 0.02-0.044, indicating that genetic divergence between them was low; Eriocheir leptognathus (population from Nanpaihe, Tianjin) was the most distant taxon with genetic distances value of 0.147-0.195, which could be defined as genetic distances between species in genus Eriocheir.

关 键 词:遗传多样性  螃蟹  细胞色素  氧化酶  基因

Genetic Differences of Mitten Crabs Based on RFLP Analysis on Mitochondrial Cytochrome Oxidase Subunit I (COI) Gene
HU Pengfei,;WANG Qian,;DAI Wei,;WANG Xiaomei.Genetic Differences of Mitten Crabs Based on RFLP Analysis on Mitochondrial Cytochrome Oxidase Subunit I (COI) Gene[J].Journal of Northeast Agricultural University,2008,15(1):51-57.
Authors:HU Pengfei  WANG Qian  DAI Wei  WANG Xiaomei
Institution:1. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture,Fisheries Science Department,Tianjin Agricultural University,Tianjin 300384,China;College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China
2. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture,Fisheries Science Department,Tianjin Agricultural University,Tianjin 300384,China
Abstract:The genetic differences of 15 mitten crab populations from 6 river systems in mainland China and 1 population from Russia were studied based on RFLP analysis of mitochondrial cytochrome oxidase subunit I (COI).The results showed that Tas I-RFLP pattern could be used as a genetic marker to distinguish Eriocheir hepuensis from Eriocheir sinensis, Eriocheirjaponica and Eriocheir leptognathus;genetic distances among 13 populations ofEriocheir sinensis range from 0 to 0.015, indicating that they were different geographic strains;the subspecies status ofEriocheir sinensis and Eriocheir hepuensis (population from Nanliujiang) were considered owning to their genetic distances of 0.02-0.044,indicating that genetic divergence between them was low; Eriocheir leptognathus (population from Nanpaihe, Tianjin) was the most distant taxon with genetic distances value of 0.147-0.195,which could be defined as genetic distances between species in genus Eriocheir.
Keywords:mitten crab  genetic differences  mitochondrial COI gene  RFLP
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