The metabolism of DDT in vivo by the Japanese quail (Coturnix coturnix japonica) |
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| Authors: | MM Ahmed CH Walker |
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| Institution: | Department of Physiology and Biochemistry, The University, Whiteknights, Reading RG6 2AJ, England |
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| Abstract: | Male and female Japanese quail (Coturnix coturnix japonica) were given intraperitoneal injections of 14C]DDT in ethanol at a rate of 13.4 mg/kg body wt. Fifty-six days later the tissues and droppings were analysed for total 14C and metabolites. The rate of loss of 14C in droppings was very similar in males and females. The maximal rate was reached on the third day, and 65–66% of the injected dose was voided by the fifty-sixth day. Ninety-three to ninety-four percent of the 14C in droppings and 83–90% of the 14C in tissues were extracted by solvents. Combined extracts from males and females were used for determination of DDT and its metabolites. Expressing all results as percentages of injected dose, the following were isolated from droppings: DDA (24%), DDT (3%), DDD (5.1%), DDE (11%), and uncharacterised polar metabolites (17%). Twenty-five percent of the dose was retained in the tissues and this was largely accounted for as DDT (10.4%) and DDE (10.5%). Of the total metabolites found 31% was DDE (almost equally divided between tissues and droppings) and 35% was DDA (almost entirely in droppings). Since DDD was not found in significant quantities in tissues, the substantial quantities in droppings were probably produced from DDT by the action of microorganisms. |
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