| 马铃薯脱外壁花粉的制备与人工萌发 |
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| 引用本文： | 吴旺泽,王蒂,王清,彭晓莉.马铃薯脱外壁花粉的制备与人工萌发[J].园艺学报,2005,32(1):39-43. |
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| 作者姓名： | 吴旺泽 王蒂 王清 彭晓莉 |
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| 作者单位： | (甘肃农业大学农学院, 兰州730070) |
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| 基金项目： | 国家高技术研究发展计划‘863’项目(2001AA241131) |
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| 摘 要： | 将3个马铃薯普通栽培种( Solanum tuberosum L. ) ‘陇薯3号’、‘Favorita’和‘Shapody’的花粉先经低温饥饿处理, 再低温水合、高温热激和渗激, 分离出大量具有活力的脱外壁花粉, 花粉最高脱壁率达34.5%。脱外壁花粉在K3 培养基+ 15% PEG + 5%蔗糖+ 0.01% H3BO3 + 0.01% LH + 100mg/L PVP的萌发液中萌发率高达38.3%。试验建立了三步冲击法制备马铃薯脱外壁花粉的方法, 并讨论了外壁在花粉萌发与受粉中的生物学意义。
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| 关 键 词： | 马铃薯 花粉 脱外壁花粉 人工萌发 |
| 文章编号： | 0513-353X(2005)01-0039-05 |
| 收稿时间： | 2004-3-11 |
| 修稿时间： | 2004-8-12 |
Preparation and Artificial Germination of De-exined Pollen in Potato(Solanum tuberosum L.) |
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| Wu Wangze,Wang Di,Wang Qing,Peng Xiaoli.Preparation and Artificial Germination of De-exined Pollen in Potato(Solanum tuberosum L.)[J].Acta Horticulturae Sinica,2005,32(1):39-43. |
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| Authors: | Wu Wangze Wang Di Wang Qing Peng Xiaoli |
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| Institution: | (College of Agronomy , Gansu Agriculture University, Lanzhou 730070, China) |
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| Abstract: | Pollen grains of three cultivars‘Longshu 3’, ‘Favorita’and ‘Shapody’of potato were treated following a three2step p rocedure: 1. Starvation treatment at 4 - 7℃ for 3 - 5 d; 2. Hydration at 4℃ for 20 h; 3. Heat shock at 36℃ for 20 - 100 min and osmotic shock for 40 min. After the three2step treatment the exine could be detached along the germination furrowswith the detachment rate of 34.5%. The germination rate of the nake pollen of cultivars potato could reach 38.3% in a modified medium containing 15% PEG (MW 8000) , 5% sucrose, 0.01% H3BO3 , 0.01% LH, 100 mg/L PVP and K3'salts ( pH 8.5). The results showed the three-step procedure established in this experiment was a useful tool for detaching the exine of potato pollen and understanding the biological role of exine in pollen germination and pollination. |
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| Keywords: | Potato Pollen De-exined pollen Artificial pollen germination |
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