| “锦绣”、“丰黄”黄桃变异株的SRAP分析 |
| |
| 引用本文： | 周平,郭瑞,雷龑,金光,廖汝玉,陈小明,黄新忠,陈由强.“锦绣”、“丰黄”黄桃变异株的SRAP分析[J].福建农业学报,2010,25(4). |
| |
| 作者姓名： | 周平 郭瑞 雷龑 金光 廖汝玉 陈小明 黄新忠 陈由强 |
| |
| 作者单位： | 1. 福建省农业科学院果树研究所,福建,福州,350013
2. 福建师范大学生命科学学院,福建,福州,350108 |
| |
| 基金项目： | 国家科技支撑计划项目，福建省科技计划重点项目，福建省公益类科研院所基本科研专项，福建省财政专项，福建省农业科学院青年科技人才创新基金 |
| |
| 摘 要： | 应用SRAP技术对"锦绣"黄桃、"丰黄"黄桃及其相应变异株系等7个材料进行了分析。从40对引物组合中筛选出2对引物在"丰黄"及变异株"锦黄"之间有明显扩增带差异,表明遗传变异已发生。分别纯化、克隆、测序这些特异条带,分析序列预测所示基因,并设计引物将其转化为稳定SCAR标记。应用特异的SCAR标记可快速检测鉴定相应变异株,进一步验证了变异的存在。试验未筛选到合适引物可区分"锦绣"及其变异株系。
|
| 关 键 词： | 黄桃 变异株 相关序列扩增多态性 特征性片断扩增区域 |
SRAP analysis on variant lines of Jinxiu and Fenghuang yellow peach |
| |
| ZHOU Ping,GUO Rui,LEI Yan,JIN Guang,LIAO Ru-yu,CHEN Xiao-ming,HUANG Xin-zhong,CHEN You-qiang.SRAP analysis on variant lines of Jinxiu and Fenghuang yellow peach[J].Fujian Journal of Agricultural Sciences,2010,25(4). |
| |
| Authors: | ZHOU Ping GUO Rui LEI Yan JIN Guang LIAO Ru-yu CHEN Xiao-ming HUANG Xin-zhong CHEN You-qiang |
| |
| Institution: | ZHOU Ping1,GUO Rui1,LEI Yan1,JIN Guang1,LIAO Ru-yu1,CHEN Xiao-ming1,HUANG Xin-zhong1,CHEN You-qiang2(1.Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China,2.College of Life Sciences,Fujian Normal University,Fujian 350108,China) |
| |
| Abstract: | Seven yellow peach samples,including Jinxiu,Fenghuang and their variant lines,were studied by means of the sequence-related amplified polymorphism(SRAP) molecular marker technology.By screening a total of 40 SRAP primer combinations,it was found that 2 combinations could positively identify the differential amplification between Fenghuang and its variant line Jinhuang,as they were genetically diversified.Subsequently,these specific marker bands were cloned,sequenced,analyzed and converted into stable sequen... |
| |
| Keywords: | yellow peach variant line SRAP SCAR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
