| Elicitin诱导烟草微敏反应和防卫基因的表达 |
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| 引用本文： | 梁元存,刘爱新,王金生,张天宇,董汉松.Elicitin诱导烟草微敏反应和防卫基因的表达[J].植物病理学报,2007,37(6):610-615. |
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| 作者姓名： | 梁元存 刘爱新 王金生 张天宇 董汉松 |
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| 作者单位： | 1 山东农业大学植物病理学系, 泰安 271018;2 南京农业大学农业部病虫检测与治理重点开放实验室, 南京 210095 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金;教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 研究了一种α型elicitin-parasiticein对烟草微敏反应(microscopic hypersensitive response, micro-HR)和防卫反应分子表征基因表达的诱导作用。用150 nmol/L的parasiticein喷洒烟草叶片12 h后, 经曲利本蓝(trypan blue)染色, 光镜下可观察到有细胞坏死, 说明parasiticein引起了micro-HR, 而水和低浓度的parasiticein不能引起micro-HR。用parasiticein注射叶片可引起肉眼可见的HR, 注射用parasiticein浓度远比喷洒引起micro-HR的浓度低。Parasiticein还可诱导对TMV的抗性, 浓度在30 nmol/L时比150 nmol/L的效果好。用parasiticein注射烟草30 min, HR表征基因hsr203J和hin1开始表达, 在9 h内逐渐降低, 12~16 h肉眼可观察到HR。Parasiticein喷洒烟草后, 病程相关蛋白(pathogenesis-related, PR)基因PR-1b在诱导的第1 d到第5 d都有一定量的表达。可见, parasiticein能同步诱导过敏反 应和抗病 性及其分 子表征基 因的表达 。
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| 关 键 词： | elicitin parasiticein 微敏反应(micro-HR) 防卫反应 |
| 文章编号： | 0412-0914（2007）06-0610-06 |
| 收稿时间： | 2006-11-03 |
| 修稿时间： | 2007-03-01 |
Microscopic hypersensitive response and expression of defense response induced by the proteinaceous elicitor elicitin-parasiticein |
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| LIANG Yuan-cun,LIU Ai-xin,WANG Jin-sheng,ZHANG Tian-yu,DONG Han-song.Microscopic hypersensitive response and expression of defense response induced by the proteinaceous elicitor elicitin-parasiticein[J].Acta Phytopathologica Sinica,2007,37(6):610-615. |
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| Authors: | LIANG Yuan-cun LIU Ai-xin WANG Jin-sheng ZHANG Tian-yu DONG Han-song |
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| Institution: | 1 Department of Plant Pathology, Shandong Agricultural University, Taian 271018, China;2 Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Elicitins produced by species of plant pathogenic oomycetes constitute a unique group of small proteins that induce hypersensitive response(HR) and pathogen defense in plants.Modes and molecular bases of the responses have not been well understood.Here we studied a particular elicitin,parasiticein from Phytophthora parasitica var.nicotianae,and focused on modes of HR and pathogen defense that the protein causes.To illustrate whether parasiticein induces microscopic hypersensitive response(micro-HR),tobacco leaves were sprayed with parasiticein,stained with trypan blue,and observed by light microscopy.Micro-HR was observed on leaves in 12 h after treatment with a moderate dose of parasiticein.Inversely,water and low concentrations of parasiticein did not cause evident micro-HR.Tobacco resistance to Tobacco mosaic virus(TMV) also was induced,as observed based on scoring leaf lesions caused by TMV inoculated to untreated leaves at 3 days after treatment.The application of 30 nmol/L parasiticein provided the optimal level of resis-tance,in comparison with those induced by a range of the elicitin concentrations.Consistently,HR marker genes hsr203J and hin1 were expressed in leaves at extents coincident with the HR levels.The defense response gene,PR-1b,was expressed concomitantly with resistance development.Moreover,PR-1b started to express by 24 h and lasted at least 5 days after parasiticein treatment,suggesting a time course of the molecular responses initiated prior to the onset of resistance.These results suggest that the elicitin induces micro-HR and pathogen defense in consistence with activation of critical marker genes. |
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| Keywords: | elicitin parasiticein |
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