| 乌头总碱的纯化工艺及中乌头碱的纯化研究 |
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| 引用本文： | 万里翔,张岳峰,侯大斌.乌头总碱的纯化工艺及中乌头碱的纯化研究[J].安徽农业科学,2008,36(12):5044-5046. |
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| 作者姓名： | 万里翔 张岳峰 侯大斌 |
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| 作者单位： | 西南科技大学生命科学与工程学院药用植物研究室,四川绵阳,621010 |
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| 基金项目： | 国家科技支撑计划
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四川省科技攻关项目 |
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| 摘 要： | 目的]选出乌头总碱的最佳提取工艺,并分离纯化中乌头碱,为新药研制奠定基础。方法]以总生物碱提取率为指标考察提取工艺。通过硅胶柱层析和结晶纯化获得纯度在95%以上的中乌头碱。结果]附子中乌头总碱的最佳提取条件为:以3倍药材量的95%乙醇溶液为溶剂,浸提3次,第1次5 d,第2次3 d,第3次1 d。调提取液pH值为3.0~4.0后静置12 h,回收乙醇并浓缩至一定体积,确定D-101的最佳吸附率、洗脱率分别为87.5%和95.3%。纯化时,D-101大孔吸附树脂的上样浓度为6 g/L,pH值为4.5,室温为18℃,以蒸馏水除杂,流速为1 ml/min的95%乙醇洗脱,浓缩洗脱液,完全蒸干,测得其附子总生物碱的平均含量高于48%。结论]D-101大孔吸附树脂可用于工业化乌头总碱的提取,以及后续各种生物碱的纯化。
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| 关 键 词： | 中乌头碱 树脂 乌头总碱 分离 纯化 |
| 文章编号： | 0517-6611(2008)12-05044-03 |
| 修稿时间： | 2008年2月22日 |
Study on the Extraction Technology of Total Aconitines and Purification of Mesaconitine |
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| WAN Li-xiang.Study on the Extraction Technology of Total Aconitines and Purification of Mesaconitine[J].Journal of Anhui Agricultural Sciences,2008,36(12):5044-5046. |
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| Authors: | WAN Li-xiang |
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| Abstract: | Objective] The aim was to select out the optimum extraction technology of total aconitines and isolate and purify mesaconitine so as to lay a foundation for preparing new medicines.Method] With the extraction rate of total alkaloid as index,the extraction technology was studied.The mesaconitine with purity higher than 95% was obtained through silica gel column chromatography and crystallization purification.Result] The optimum extraction condition of total aconitines from aconite was as follows: with 3 times amount of 95% ethanol as solvent,extraction for 3 times and for 5 d at the 1st,3 d at the 2nd and 1 d at the 3rd.The extracts was stationary for 12 h since its pH value was adjusted to be 3.0～4.0,the ethanol was recycled and concentrated to a certain volume,the optimum adsorption and elution rates of D-101 were ascertained to be 87.5% and 95.3%.During purification,the sample concn.of D-101 macroporous adsorption resin was 6 g/L,the pH value was 4.5,the room temperature was 18 ℃,the impurities were removed by distilled water,the sample was eluted by 95% ethanol with flow velocity of 1 ml/min,the eluent was concentrated,till dried out completely and the determined average content of total alkaloid from aconite was higher than 48%.Conclusion] D-101 macroporous adsorption resin could be used in the industrial extraction of total aconitines and subsequent purifications of various alkaloids. |
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| Keywords: | Mesaconitine Resin Total aconitines Isolation Purification |
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