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Characterisation of imidacloprid resistance mechanisms in the brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae) |
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| Authors: | Alin M Puinean Ian Denholm Ralf Nauen |
| Institution: | a Centre for Pest & Disease Management, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK b Department of Neuroscience Physiology & Pharmacology, University College London, London WC1E 6BT, UK c Bayer CropScience AG, Research Insecticides, Alfred Nobel Str. 50, D-40789 Monheim, Germany |
| Abstract: | Effective control of the brown planthopper, Nilaparvata lugens Stål, across rice-growing regions of Asia has been seriously compromised over the last 2 years by the appearance of widespread resistance to the neonicotinoid insecticide, imidacloprid. Sequence analysis of the ligand-binding domain of the nicotinic acetylcholine receptor α1 subunit from two field-collected resistant strains (CHN-2 and IND-11) did not reveal the Y151S point mutation previously implicated in conferring target-site resistance in this species. This result was supported by ligand-binding studies with 3H]-imidacloprid that showed no significant change in insecticide binding to isolated membranes from susceptible and resistant strains. In contrast, there was an approximate 5-fold increase in the mixed function oxidase activity for the two resistant strains suggesting that imidacloprid metabolism by increased cytochrome P450 monooxygenase activity is the major mechanism of resistance in these strains. |
| Keywords: | Nilaparvata lugens Neonicotinoid Imidacloprid Metabolic resistance Oxidase activity |
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