| 山东省不同年代栽培大豆SSR标记遗传多样性分析 |
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| 作者姓名： | 韩雪芹 林延慧 张礼凤 蒿燕 张丽娟 |
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| 作者单位： | [1]青岛农业大学植物科技学院作物遗传育种实验室,青岛266109 [2]山东省农业科学院作物研究所,济南250100 |
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| 摘 要： | 从分子水平探讨山东省大豆种质资源遗传多样性，为山东大豆今后的大豆育种和遗传改良奠定基础；对山东省36份不同年代材料进行SSR标记，计算遗传相似系数，并对供试品种进行UPGMA聚类分析；20对SSR引物共扩增出138个等位变异，平均每对引物扩增出6．9个等位变异。遗传相似系数的变化范围为0．66-0．97，总体平均值为0．78；相似系数大，品种间遗传差异较小。利用SSR标记的数据结果，对供试品种进行聚类分析，东解1号单独聚为一类，其他品种聚成2个类群，聚类结果表明其多样性与地理来源及系谱关系相关联，但从年代上并没有很好的划分。对山东大豆的多样性分析也有必要采取多种方法对其进行综合有效的鉴定。
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| 关 键 词： | 山东大豆 SSR 遗传多样性 山东省 栽培大豆 遗传多样性分析 Different Cultivars Soybean Shandong Genetic Diversity 综合 方法 划分 关联 关系 系谱 地理来源 聚类分析 类群 结果 数据 利用 |
Analysis of Genetic Diversity by SSR for Shandong Soybean Cultivars in Different Years |
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| Authors: | Han Xueqin Lin Yanhui Zhang Lifeng Hao Yan Zhang Lijuan |
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| Abstract: | In order to establish the foundation for Shandong soybean breeding and genetic improvement, genetic diversity of Shandong soybeans were discussed on the molecular level. 36 summer soybean cultivars in Shandong province of different years were analyzed method according to the genetic similarity coefficient. by SSR marker and cluster analysis with the UPGMA In this research, there are 138 alleles were amplified by 20 SSR primers and with the mean of 6.9. The distribution of genetic similarity coefficient was 0.66-0.97 and mean 0.78. The similarity coefficient was higher and the difference among the cultivars was lower. Integrating the data of SSR, with clustering analysis, these varieties were clustered into tow groups except Dongjie 1 hao, which indicated that the genetic diversity is related to their regions and genealogy. But the division on years was not clear. It was advised that it should also been analyzed by many kinds of methods for the genetic diversity analysis of Shandong soybeans. |
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| Keywords: | Shandong soybean eultivars SS-R genetic diversity |
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