外源ABA和乙烯利胁迫下斑茅ACO基因表达的实时PCR分析 |
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作者姓名: | 郭春芳 张积森 张木清 陈如凯 |
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作者单位: | [1]福建农林大学农业部甘蔗生理生态与遗传改良重点实验室,福州350002 [2]福建教育学院,福州350025 [3]福建师范大学生命科学学院,福州350108 |
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基金项目: | 国家自然科学基金
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高等学校博士学科点专项科研项目 |
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摘 要: | 研究了外源ABA和乙烯利胁迫处理对斑茅1-氨基环丙烷-1-羧酸氧化酶基因(ACO基因)表达情况的影响。在项目组克隆斑茅ACO基因的基础上,应用实时荧光PCR技术分析斑茅ACO基因在ABA、乙烯利胁迫下的表达。结果表明,斑茅ACO基因在ABA的胁迫下,在3h有微弱上调表达,而6h日寸明显抑制,其后表达趋向平缓;在乙烯利的胁迫下,ACO基因在前期受抑制,其后呈明显上调表达,在24h后ACO基因的表达趋向平缓。斑茅ACO基因的表达受乙烯利诱导,而不受外源ABA诱导。
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关 键 词: | 斑茅 1-氨基环丙烷-1-羧酸氧化酶基因(ACO 基因) 脱落酸 乙烯利 实时荧光PCR 乙烯利 胁迫处理 斑茅 基因表达 技术分析 Realtime Stress Ethephon Exogenous ABA Gene Expression 平缓 上调表达 结果 实时荧光 应用 克隆 项目组 影响 情况 |
Analysis of The Expression of ACO Gene Under Exogenous ABA and Ethephon Stress in Erianthus arundinaceus throught Realtime PCR |
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Authors: | Guo Chunfang Zhang Jisen Zhang Muqing Chen Rukai |
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Abstract: | In order to study the molecular mechanism of A CO gene from Erianthus arundinaceum under exogenous ABA and ethephon stress. In this paper, based on the cloning of A CO gene in Erianthus arundi- naceus, the expression of A CO gene in Erianthus arundinaceus under ABA and ethephon treatments was investigated by Real-time fluorescence PCR. The results showed that the expression of A CO gene was slightly up-regulated at the beginning and continuous inhibited, then became normal by ABA stress . Under the treatment of ethephon, the expression of A CO gene was prohibited at the earlier stage along with the subsequent continuous more obvious up-regulation. The results showed that the expression of A CO gene in Erianthus arundinaceus could be induced by ethephon, but not by ABA. |
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