| 牛病毒性腹泻病病毒NS3表位串联蛋白表达及抗体间接ELISA方法的建立 |
| |
| 引用本文： | 贾莹,李岩,尹鑫,温凯,刘华,张文龙,王君伟.牛病毒性腹泻病病毒NS3表位串联蛋白表达及抗体间接ELISA方法的建立[J].畜牧兽医学报,2011,42(8). |
| |
| 作者姓名： | 贾莹 李岩 尹鑫 温凯 刘华 张文龙 王君伟 |
| |
| 作者单位： | 东北农业大学动物医学学院,哈尔滨,150030 |
| |
| 基金项目： | 现代农业产业技术体系建设专项资金(NYCYTX-0303) |
| |
| 摘 要： | 为建立一种有效的牛病毒性腹泻病病毒(Bovine viral diarrhea virus,BVDV)抗体检测方法,作者将BVDVNS3蛋白2个抗原表位的编码核酸序列进行串联,构建重组表达载体pET-30a-EXnN,并在原核表达系统中表达了重组蛋白。选取包含12个表位肽的重组蛋白(r-BVDV-EX6N)作为包被抗原建立NS3蛋白抗体间接ELISA方法。该方法的特异性和稳定性良好,与2种商品化试剂盒的符合率分别为96.05%和78.95%。试验结果表明建立的ELISA方法可用于BVDV NS3蛋白抗体的检测。
|
| 关 键 词： | BVDV NS3蛋白 表位串联 间接ELISA |
Development of Indirect ELISA of Bovine Viral Diarrhea Virus by Using Tandem Epitopes of NS3 |
| |
| JIA Ying,LI Yan,YIN Xin,WEN Kai,LIU Hua,ZHANG Wen-long,WANG Jun-wei.Development of Indirect ELISA of Bovine Viral Diarrhea Virus by Using Tandem Epitopes of NS3[J].Acta Veterinaria et Zootechnica Sinica,2011,42(8). |
| |
| Authors: | JIA Ying LI Yan YIN Xin WEN Kai LIU Hua ZHANG Wen-long WANG Jun-wei |
| |
| Institution: | JIA Ying,LI Yan,YIN Xin,WEN Kai,LIU Hua,ZHANG Wen-long,WANG Jun-wei (College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China) |
| |
| Abstract: | The present experiment was performed to establish an indirect ELISA for detection of antibodies against Bovine viral diarrhea virus(BVDV).Tandem epitopes of NS3 of BVDV was cloned into pET-30a and expressed in E.coli Rosetta.SDS-PAGE analysis revealed a band of protein correspondent with molecular weight of the target protein,and the result of western blot showed that the recombinant protein was recognized specifically by anti-BVDV positive serum.An indirect ELISA(r-BVDV-EX6N-ELISA)was developed using purif... |
| |
| Keywords: | Bovine viral diarrhea virus(BVDV) NS3 protein tandem epitopes indirect ELISA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
